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Isolera system

Manufactured by Biotage
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Sourced in Sweden

The Isolera system is a high-performance flash chromatography purification system designed for efficient separation and purification of chemical compounds. It utilizes automated flash chromatography to enable rapid and reliable purification of a wide range of compounds.

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Lab products found in correlation

Nicolet is5 ftir spectrometer, by Thermo Fisher Scientific (1 mentions) P 2000, by Jasco (1 mentions) Initiator microwave system, by Biotage (1 mentions) Topspin software, by Bruker (1 mentions)

Close spelling variants of this product

Isolera™ Spektra Systems Isolera One Flash Chromatography System Isolera chromatography system Isolera purification system Isolera 1 System Isolera FLASH purification system Isolera flash chromatography system Isolera One system Isolera Prime system Isolera One purification system

7 protocols using isolera system

1

Comprehensive Analytical Characterization of Compounds

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The optical rotation was measured by a polarimeter JASCO P-2000 (JASCO, Tokyo, Japan). The infrared spectra were obtained on a FT-IR spectrophotometer, Nicolet™ iS™ 5 FTIR Spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). UV spectra were collected by Spectrophotometer U-3310 UV-Vis (Hitachi, Ltd., Tokyo, Japan). The 1D and 2D NMR spectra were obtained on an Agilent 600 MHz DD2 NMR (Agilent, Santa Clara, CA, USA). The chloroform-d was used as the internal lock. HRESIMS data in positive mode were collected on a Waters LC/Q-TOF SYNAPT G2 (Waters Corporation, Milford, MA, USA) system. All isolations were purified by MPLC and HPLC. The former is Biotage® Isolera™ Systems (Biotage, Uppsala, Sweden), and the powder was filled in the flash column, Biotage® SNAP Cartridge KP-Sil 10 g (Biotage, Uppsala, Sweden), the latter is HPLC system Shimazu LC-2050 (Shimazu, Kyoto, Japan) with a Galaksil column EF-C18-H (5 μm, 120 Å, 10 × 250 mm, C18; Galak Chromatography, Wuxi, Jiangsu, China).
Nguyen N.B., Chen L.Y., Chen P.J., El-Shazly M., Hwang T.L., Su J.H., Su C.H., Yen P.T., Peng B.R, & Lai K.H. (2022). MS/MS Molecular Networking Unveils the Chemical Diversity of Biscembranoid Derivatives, Neutrophilic Inflammatory Mediators from the Cultured Soft Coral Sarcophyton trocheliophorum. International Journal of Molecular Sciences, 23(24), 15464.
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2

Piperine Metabolite Purification and Characterization

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Piperine metabolites were purified by flash column chromatography Biotage® Isolera™ Systems (Uppsala, Sweden). DCM 100% was used to remove electrolytes from the compound mixture or by recrystallization of TBAPF6 using methanol, and the solvent combination (SiO2, eluent—DCM: isopropanol—98:2) was used to afford the title compound. The product was dissolved in CDCl3 (0.6 mL) with a tetramethylsilane (TMS) reference, and 1H and 13C NMR spectra were obtained.
Asra R., Povinelli A.P., Zazeri G, & Jones A.M. (2024). Computational Predictive and Electrochemical Detection of Metabolites (CP-EDM) of Piperine. Molecules, 29(10), 2406.
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3

Synthesis of Oxaliplatin-Capecitabine Conjugate

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Oxaliplatin(IV)(Cape)2 (PTC) was prepared as follows: 170 mg (0.238 mmol; 1.00 eq.) of oxaliplatin(IV)(MSC)2 was solubilized in 2 mL of anhydrous DMSO together with 214 mg (0.596 mmol; 2.50 eq.) of capecitabine and 29 mg (0.24 mmol; 1.0 eq.) of 4-(dimethylamino)pyridine. The mixture was stirred overnight, and then diethyl ether was added until the formation of an insoluble oil. The supernatant was removed, and the oil was solubilized in 0.5 mL of methanol. The crude product was recovered through precipitation with diethyl ether. The product (91 mg; yield 32%) was obtained through purification on an Isolera system for flash column chromatography on reverse-phase C18 silica (Biotage, Uppsala, Sweden). The elution was performed through an elution gradient with methanol and water.
1HNMR (400 MHz, DMSO-d6) δ: 8.55 (m; 2H; carbamate group), 8.03 (m; 2H; aromatic), 7.07 (m; 4H; amino group), 3.58–0.84 (unattributable multiplets). Elemental analysis: calc. C 39.97%, H 4.70%, N 9.32%; exp. C 39.48%, H 5.12%, N 9.73%.
Marotta C., Cirri D., Kanavos I., Ronga L., Lobinski R., Funaioli T., Giacomelli C., Barresi E., Trincavelli M.L., Marzo T, & Pratesi A. (2024). Oxaliplatin(IV) Prodrugs Functionalized with Gemcitabine and Capecitabine Induce Blockage of Colorectal Cancer Cell Growth—An Investigation of the Activation Mechanism and Their Nanoformulation. Pharmaceutics, 16(2), 278.
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4

Purification of Crude Extract via Flash Chromatography

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The crude extract (∼4 g from 30 L culture) was absorbed onto silica gel and subjected to flash chromatography fractionation using a hexane-CH2Cl2-MeOH gradient on an Isolera System (Biotage, Charlotte, NC). The purification of each compound was achieved as follows:
Theodore C.M., Stamps B.W., King J.B., Price L.S., Powell D.R., Stevenson B.S, & Cichewicz R.H. (2014). Genomic and Metabolomic Insights into the Natural Product Biosynthetic Diversity of a Feral-Hog-Associated Brevibacillus laterosporus Strain. PLoS ONE, 9(3), e90124.
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5

Synthesis of 2-(4-Methoxyphenyl)-3-Dimethylaminoacrylic Acid Methyl Ester

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Into a 20 mL microwave vial eqipped with magnetic stir bar and crimping cap was added 2-(4-methoxy-phenyl)-3dimethylaminoacrylic acid methyl ester (580 mg, 2.46 mmol), acetic acid (7 mL) and acetic anhydride (3 mL). The resulting mixture was heated in a Biotage Initiator Microwave system for 1 hour at 100°C. The reaction mixture was diluted with a water (25 mL)/ethyl acetate (20 mL) mixture and the phases were separated. The aqueous phase was extracted with additional ethyl acetate (2×20 mL) and the combined organic phases were washed with saturated, aqueous sodium bicarbonate and dried over anhydrous sodium sulfate. The drying agent was filtered off and the solvent was removed in vacuo to give a yellow oil (572 mg, 92.7% yield). An analytical sample was prepared by flash chromatography on a Biotage Isolera system, resulting in an oil, which exhibited the following properties: bp 150°C at 0.330 torr; 1H NMR (CDCl, 500 MHz) δ 8.45 (s, 1H), 7.30 (d, J = 8.8 Hz, 2H), 6.94 (d, J = 8.8 Hz, 2H), 3.85 (s, 3H), 3.82 (s, 3H) and 2.18 (s, 3H); 13C NMR (CDCl3, 75 MHz) δ 167.3, 166.9, 159.2, 143.8, 131.1, 123.8, 117.6, 113.4, 55.2, 52.0 and 20.6; IR (neat) 1776 and 1710 cm−1; HRMS (ES, M+Na) m/z calcd for C13H14O5Na 273.0733 found 273.0752.
Gupton J.T., Crawford E., Mahoney M., Clark E., Curry W., Lane A., Shimozono A., Moore-Stoll V., Elofson K., Juekun W., Newton M., Yeudall S., Jaekle E., Kanters R, & Sikorski J.A. (2018). Application of vinylogous carbamates and vinylogous aminonitriles to the regiospecific synthesis of uniquely functionalized pyrroles and quinolones. Tetrahedron, 74(52), 7408-7420.
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6

Purification and Characterization of Organic Compounds

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All materials were purchased from commercial suppliers and used without further purification. Pre-purification and QC analyses were done on a Waters Acquity UPLC/PDA/ELSD/MS system carried out with a BEH C18 2.1 x 50 mm column using gradient elution. Purification of compounds was carried out by normal phase column chromatography using pre-pack SNAP silica cartridges on a Biotage Isolera system. Reported yields were not optimized. Structures were determined by NMR spectroscopy and purity was determined by LC-MS/ELSD. NMR spectra (1D 1H and 13C, respectively, and 2D 1H-13C HSQC and 1H-13C HMBC, respectively) were recorded on a Bruker 600 MHz spectrometer equipped with TCI cryogenic gradient probe and processed and analyzed using Bruker Topspin software.
Iconaru L.I., Das S., Nourse A., Shelat A.A., Zuo J, & Kriwacki R.W. (2021). Small molecule sequestration of the intrinsically disordered protein, p27Kip1, within soluble oligomers. Journal of molecular biology, 433(18), 167120.
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7

Synthesis of Oxaliplatin-Gemcitabine Conjugate

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Oxaliplatin(IV)(Gem)2 (PTG) was prepared as follows: 455 mg (0.770 mmol; 2.50 eq.) of MSC-di-Boc-gemcitabine and 140 mg (0.320 mmol; 1.00 eq.) of oxaliplatin(IV)(OH)2 were solubilized in 2 mL of DMSO. The mixture was stirred overnight, and then diethyl ether was added until the formation of an insoluble oil. The supernatant was removed, and the crude product was solubilized in 2 mL of dichloromethane. Subsequently, 1 mL of TFA was added, and the solution was stirred at room temperature for 45 min. The solution was then dried through a rotary evaporator, and the residual oil was solubilized with about 0.5 mL of methanol. The crude product was recovered through precipitation with diethyl ether. The product (184 mg; yield 56%) was obtained through purification on an Isolera system for flash column chromatography on reverse-phase C18 silica (Biotage, Uppsala, Sweden). The elution was performed through an elution gradient with methanol and water.
1HNMR (400 MHz, DMSO-d6) δ: 8.43 (m; 4H; amino group), 7.84 (m; 4H; amino group), 7.48 (m; 2H; aromatic), 7.38 (m; 2H; aromatic), 6.12 (b; 2H; sugar), 5.75 (m; 2H; sugar), 4.37–3.82 (m; 6H; sugar), 2.05 (m; 2H; DACH), 1.47 (m; 4H; DACH), 1.08 (m; 2H; DACH). Elemental analysis: calc. C 33.31%, H 3.39%, N 11.10%; exp. C 32.87%, H 2.91%, N 10.82%.
Marotta C., Cirri D., Kanavos I., Ronga L., Lobinski R., Funaioli T., Giacomelli C., Barresi E., Trincavelli M.L., Marzo T, & Pratesi A. (2024). Oxaliplatin(IV) Prodrugs Functionalized with Gemcitabine and Capecitabine Induce Blockage of Colorectal Cancer Cell Growth—An Investigation of the Activation Mechanism and Their Nanoformulation. Pharmaceutics, 16(2), 278.
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