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Ciglitazone

Manufactured by Cayman Chemical
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Ciglitazone is a chemical compound used as a research tool in scientific laboratories. It is a peroxisome proliferator-activated receptor gamma (PPARγ) agonist. Ciglitazone can be used to study the biological effects of PPARγ activation.

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Lab products found in correlation

Gw9662, by Cayman Chemical (6 mentions) Rosiglitazone, by Cayman Chemical (4 mentions) Pioglitazone, by Cayman Chemical (4 mentions) Troglitazone, by Cayman Chemical (4 mentions) 15d pgj2, by Cayman Chemical (2 mentions) Propidium iodide, by Merck Group (2 mentions) N acetylcysteine, by Merck Group (2 mentions) Z lehd fmk, by Merck Group (1 mentions) Tiron, by Merck Group (1 mentions) 15 deoxy δ12 14 prostaglandin j2 pgj2, by Cayman Chemical (1 mentions) Isoliquiritigenin, by Cayman Chemical (1 mentions) N acetyl l cysteine nac, by Abcam (1 mentions) Bezafibrate, by Cayman Chemical (1 mentions) Fenofibrate, by Cayman Chemical (1 mentions) Diphenyleneiodonium, by Merck Group (1 mentions) Mcc950, by Cayman Chemical (1 mentions) 3 isobutyl 1 methylxanthine ibmx, by Merck Group (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Insulin, by Merck Group (1 mentions) Isoproterenol, by Merck Group (1 mentions)

10 protocols using ciglitazone

1

Apoptosis Induction by PPARγ Antagonists

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Thiazolidinediones (rosiglitazone, pioglitazone, ciglitazone) and the PPARγ antagonist GW9662 were provided from Cayman Chemical (Ann Arbor, MI, USA). Soluble recombinant TRAIL, proteasome inhibitor MG132, caspase 9 specific inhibitor (Z-LEHD-FMK) and propidium iodide (PI) were purchased from Sigma (Saint Quentin Fallavier, France). Caspase 8 inhibitor (Z-IETD-FMK) was from Bachem (Heidelberg, Germany). Anti-DR4 and DR5 blocking antibodies were from Alexis Biochemicals (Lausanne, Switzerland).
Plissonnier M.L., Fauconnet S., Bittard H., Mougin C., Rommelaere J, & Lascombe I. (2017). Cell death and restoration of TRAIL-sensitivity by ciglitazone in resistant cervical cancer cells. Oncotarget, 8(64), 107744-107762.
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2

Peroxisome Proliferator-Activated Receptor Agonists and Antioxidants

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Peroxisome proliferator-activated receptor (PPAR) agonists fenofibrate, troglitazone, bezafibrate, 15-deoxy-Δ12,14-Prostaglandin J2 (PGJ2), ciglitazone, rosiglitazone, pioglitazone, and MCC-555 were from Cayman Chemical (Ann Arbor, MI, USA). Antioxidants 2,2,5,7,8-Pentamethyl-6-chromanol (PMC), 4-hydroxy-TEMPO (TEMPO, a superoxide dismutase mimic) and Tiron were from Sigma (St. Louis, MO), MCI-186 (MCI), Trolox and DL-a-lipoic acid (lipoic acid) were from Cayman Chemical, N-Acetyl-L-cysteine (NAC) was from Abcam (Cambridge, MA), and elamipretide (ELAM) was from Muse Chem (Fairfield, NJ). Trolox amide analogs (669, 738, 829) were from ChemBridge (San Diego, CA). Inflammasome inhibitors MCC950 and Isoliquiritigenin were from Cayman Chemical. The NADPH-oxidases inhibitor apocynin (APO) was from Abcam and diphenyleneiodonium (DPI) was from Sigma.
Gnanaguru G., Mackey A., Choi E.Y., Arta A., Rossato F.A., Gero T.W., Urquhart A.J., Scott D.A., D’Amore P.A, & Ng Y.S. (2021). Discovery of sterically-hindered phenol compounds with potent cytoprotective activities against ox-LDL–induced retinal pigment epithelial cell death as a potential pharmacotherapy. Free radical biology & medicine, 178, 360-368.
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3

Adipocyte Differentiation Pathway Modulators

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3-Isobutyl-1-methyl-xanthine (IBMX), insulin, dexamethasone, and isoproterenol were purchased from Sigma Chemical Co. l-N5-(1-lminoethyl) ornithine dihydrochloride (L-NIO), KT5720, ODQ, wortmannin, and diethylamine NONOate (DeaNONOate) were from CALBIOCHEM, troglitazone, GW9662, and ciglitazone were from CAYMAN CHEMICAL COMPANY, and auranofin was from Alexis Biochemicals.
Yamada Y., Eto M., Ito Y., Mochizuki S., Son B.K., Ogawa S., Iijima K., Kaneki M., Kozaki K., Toba K., Akishita M, & Ouchi Y. (2015). Suppressive Role of PPARγ-Regulated Endothelial Nitric Oxide Synthase in Adipocyte Lipolysis. PLoS ONE, 10(8), e0136597.
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4

Modulating PPARγ Signaling Pathways

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All PPARγ ligands and antagonist: Rosiglitazone (10μM), GW9662 (10μM) pioglitazone (10μM), troglitazone (10μM) and ciglitazone (10μM) (Cayman chemical) were prepared in DMSO following the manufacturer’s recommendations and diluted in RPMI. Sodium butyrate (But, 2mM) was from Sigma-aldrich.
Couvigny B., de Wouters T., Kaci G., Jacouton E., Delorme C., Doré J., Renault P., Blottière H.M., Guédon E, & Lapaque N. (2015). Commensal Streptococcus salivarius Modulates PPARγ Transcriptional Activity in Human Intestinal Epithelial Cells. PLoS ONE, 10(5), e0125371.
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5

PPARβ/δ and PPARγ Agonists in Cell Assays

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The PPARβ/δ activator GW501516 was provided by Enzo Life Sciences (Villeurbanne, France). Ciglitazone (PPARγ agonist) was purchased from Cayman Chemical (Ann Arbor, MI). The final concentration of organic solvent (dimethylsulfoxide) was less than 0.1% which had no effect on cell viability.
Elie-Caille C., Lascombe I., Péchery A., Bittard H, & Fauconnet S. (2020). Molecular and nanoscale evaluation of N-cadherin expression in invasive bladder cancer cells under control conditions or GW501516 exposure. Molecular and Cellular Biochemistry, 471(1), 113-127.
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6

Quantification of Lipid Mediators

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Ammonium acetate was from Sigma Aldrich (St. Louis, MO). HPLC-grade solvents acetonitrile and methanol were from Fisher Scientific (Pittsburgh, PA, USA) and VWR (Radnor, PA, USA). Deionized water was generated in-house. PGA2, PGD2, PGE2, PGF2α, PGJ2, 15d-PGJ2, 15d-PGJ2-d4, NS-398, CAY-10404, ciglitazone, troglitazone and staurosporine were from Cayman Chemical. ABT-263 was from Apex Biotechnology. The dihomo-15d-PGJ2 standard was synthesized by Jubilant Chemsys (Bangalore, India). TRAIL was from Abcam.
Wiley C.D., Sharma R., Davis S.S., Lopez-Dominguez J.A., Mitchell K.P., Wiley S., Alimirah F., Kim D.E., Payne T., Rosko A., Aimontche E., Deshpande S.M., Neri F., Kuehnemann C., Demaria M., Ramanathan A, & Campisi J. (2021). Oxylipin biosynthesis reinforces cellular senescence and allows detection of senolysis. Cell metabolism, 33(6), 1124-1136.e5.
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7

Hepatitis B Virus Entry Inhibitor Screening

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Troglitazone was purchased from AdooQ BioScience. Ciglitazone, mitoglitazone, and trolox were purchased from Cayman Chemical. Rosiglitazone, pioglitazone, darglitazone, netoglitazone, and SR-202 were purchased from Sigma-Aldrich. Balaglitazone was purchased from MedChemExpress. Inolitazone was purchased from ChemScene. Bafilomycin A1 was purchased from Wako. A preS1 peptide consisting of aa 2-48 of the HBV preS1 region with amino-terminal myristoylation, as well as carboxy-terminal 6-carboxytetramethylrhodamine (TAMRA)-conjugated derivatives thereof, were synthesized by Scrum, Inc. The NTCP peptide fragment library was synthesized by Sigma-Aldrich. Entecavir was purchased from Santa Cruz Biotechnology.
Fukano K., Tsukuda S., Oshima M., Suzuki R., Aizaki H., Ohki M., Park S.Y., Muramatsu M., Wakita T., Sureau C., Ogasawara Y, & Watashi K. (2019). Troglitazone Impedes the Oligomerization of Sodium Taurocholate Cotransporting Polypeptide and Entry of Hepatitis B Virus Into Hepatocytes. Frontiers in Microbiology, 9, 3257.
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8

Apoptosis Regulation by PPAR Modulators

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Propidium iodide (PI), diphenyleneiodonium (DPI), and antioxidants-including N-acetyl cysteine (NAC) were purchased from Sigma-Aldrich (St. Louis, MO). Ciglitazone and the selective PPARγ antagonist GW9662 were obtained from Cayman (Ann Arbor, MI). The PPAR agonist candidate CB11 was obtained from ChemBridge. DCF-DA (2′, 7′-dichlorodihydrofluorescein diacetate) was obtained from Invitrogen (Carlsbad, CA). The caspase inhibitor, z-VAD-fmk, the caspase-3 inhibitor, z-DEVD-fmk and the caspase-9 inhibitor, z-LEHD-fmk were obtained from Calbiochem (LaJolla, CA). The ATM inhibitors, KU60019 and KU55933, were purchased from calbiochem.
Kim T.W., Hong D.W., Park J.W, & Hong S.H. (2020). CB11, a novel purine-based PPARɣ ligand, overcomes radio-resistance by regulating ATM signalling and EMT in human non-small-cell lung cancer cells. British Journal of Cancer, 123(12), 1737-1748.
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9

Receptor Binding Assay Protocols

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SP, HK-1, and NKA were purchased from Sigma Chemical Company. L732138 and GR159897 were purchased from Tocris. Rosiglitazone, ciglitazone, pioglitazone, 15d-PGJ2 and GW 9662 were purchased from Cayman Chemicals.
Levick S.P., Brower G.L, & Janicki J.S. (2019). Substance P-Mediated Cardiac Mast Cell Activation: An In Vitro Study. Neuropeptides, 74, 52-59.
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10

Apoptosis Assay with Antioxidants

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Propidium iodide (PI) and antioxidants, including N-acetylcysteine (NAC), diphenyleneiodonium (DPI, a Nox inhibitor), and apocynin (Apo, an ROS inhibitor) were purchased from Sigma Chemical (St. Louis, MO USA). Ciglitazone and the selective PPARɣ antagonist, GW9662, were obtained from Cayman (Ann Arbor, MI). PPZ023 was synthesized in the Department of Chemistry at the College of National Science, Kongju National University (Gongju, Korea). PPZ023 was dissolved in dimethyl sulfoxide (DMSO) as a 1 mM stock solution. Annexin V-FITC kits were purchased from Biovision (Palo Alto, CA, USA).
Kim T.W., Hong D.W., Kang C.M, & Hong S.H. (2020). A novel PPARɣ ligand, PPZ023, overcomes radioresistance via ER stress and cell death in human non-small-cell lung cancer cells. Experimental & Molecular Medicine, 52(10), 1730-1743.
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