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Detectx corticosterone eia kit

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The DetectX Corticosterone EIA Kit is a quantitative competitive enzyme immunoassay designed to measure corticosterone levels in biological samples. The kit provides a simple, direct, and sensitive method for the determination of corticosterone concentrations.

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Corticosterone EIA Kit (12 citations) Corticosterone (9 citations) EIA kit (4 citations) Corticosterone EIA (2 citations)

6 protocols using detectx corticosterone eia kit

1

Plasma Corticosterone Analysis in Pregnant Rats

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Pregnant dams at GD14 and GD18 were sacrificed by decapitation and trunk blood was collected using Safe-T-Fill EDTA capillary tubes (Ram Scientific Inc. #077051) and plasma was separated by centrifugation at 1000 ×g for 10 min at 4 °C, and stored at –80 °C until use in the corticosterone assay. Corticosterone (CORT) levels in plasma were determined using the DetectX Corticosterone EIA Kit (Arbor Assays, Ann Arbor, MI; #K014-H1) per the manufacturer's instructions. Optical density absorbance was determined by an Infinite M200 instrument and data analyzed using 4PLC ReaderFit online software.
Caldwell K.E., Labrecque M.T., Solomon B.R., Ali A, & Allan A.M. (2014). Prenatal arsenic exposure alters the programming of the glucocorticoid signaling system during embryonic development. Neurotoxicology and teratology, 47, 66-79.
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2

Corticosterone Quantification in Serum

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Blood was centrifuged at 5000 rpm for 15 min and serum was isolated and stored at −20°C until assayed. Serum (4 μL) was diluted 1:100–1:400 and assayed using DetectX Corticosterone EIA kit (K014; Arbor Assays, Ann Arbor, MI), per Luo et al. [4 (link)]. Intra-assay and interassay %CVs were 1.8% and 4.4%, respectively. Assay sensitivity was 18.6 pg/mL.
Kreisman M.J., McCosh R.B., Tian K., Song C.I, & Breen K.M. (2019). Estradiol enables chronic corticosterone to inhibit pulsatile LH secretion and suppress Kiss1 neuronal activation in female mice. Neuroendocrinology, 110(6), 501-516.
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3

Fecal Corticosterone Metabolite Extraction

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Fecal samples collected at day 1 (pi) followed by 1, 2, 4, 6, 8, 10, 12, 14 and 16 weeks pi were thawed, mixed, and dried at 103 °C overnight. After cooling to room temperature, samples were ground to a fine powder. Corticosterone metabolites were extracted using methods recommended by the DetectX Corticosterone EIA kit manufacturer (Arbor Assays, Ann Arbor, USA). The concentration of fecal corticosterone metabolites (FCM) was measured by DetectX Corticosterone EIA kit as per manufacturer instruction.
Sharma P., Pande V.V., Moyle T.S., McWhorter A.R, & Chousalkar K.K. (2017). Correlating bacterial shedding with fecal corticosterone levels and serological responses from layer hens experimentally infected with Salmonella Typhimurium. Veterinary Research, 48, 5.
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4

Corticosterone Measurement in Rodents

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Plasma corticosterone was measured as described (Goggin et al., 2012 (link)) using a DetectX Corticosterone EIA Kit (Arbor Assays, Ann Arbor, MI; #K014-H1). Blood was collected at 0800 h, at the start of the dark cycle and 18-hours following removal of the saccharin or saccharin plus ethanol drinking tubes.
Caldwell K.K., Goggin S.L., Labrecque M.T, & Allan A.M. (2015). The impact of prenatal alcohol exposure on hippocampal-dependent outcome measures is influenced by prenatal and early-life rearing conditions. Alcoholism, clinical and experimental research, 39(4), 631-639.
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5

Assessing Corticosterone Levels in Rodents

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Exposure to 2,3,5-trimethyl-3-thiazoline (TMT) (Contech, Victoria, BC, Canada; #300000368) was performed as previously described (Goggin et al., 2012 (link)). Animals were individually transferred to an exposure cage containing filter paper saturated with 100 μL of 3% (v/v) TMT for 10 min. Group-housed animals were exposed concurrently in parallel and returned to home cages for 20 min after the 10-min exposure. Several cohorts of animals were exposed to TMT, some of which were placed into the forced swim task 20 min after exposure, while others were rapidly decapitated for trunk blood collection using Safe-t-Fill EDTA capillary tubes. Samples were centrifuged at 1000 × g for 10 min at 4 °C. Plasma was removed and stored at −80 °C. Corticosterone (CORT) levels in plasma were determined using the DetectX Corticosterone EIA Kit (Arbor Assays, K014-H1) per manufacturer instructions with a 1:100 dilution of plasma prior to analysis. Optical density absorbance was determined by an Infinite M200 instrument and data were analyzed using 4PLC ReaderFit online software.
Tyler C.R., Solomon B.R., Ulibarri A.L, & Allan A.M. (2014). Fluoxetine treatment ameliorates depression induced by perinatal arsenic exposure via a neurogenic mechanism. Neurotoxicology, 44, 98-109.
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6

Corticosterone Concentration Determination

Cited 1 time
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Plasma corticosterone concentrations were assessed as described84 (link), employing the DetectX Corticosterone EIA Kit (Arbor Assays, Ann Arbor, MI; #K014-H1). Data were analyzed using a Four Parameter Logistic (4PL) curve fit program (Arbor Assays, https://www.myassays.com/four-parameter-logistic-curve.assay).
Caldwell K.K., Solomon E.R., Smoake J.J., Djatche de Kamgaing C.D, & Allan A.M. (2018). Sex-specific deficits in biochemical but not behavioral responses to delay fear conditioning in prenatal alcohol exposure mice. Neurobiology of learning and memory, 156, 1-16.
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