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Bim-PE is a laboratory reagent product from Cell Signaling Technology. It is a fluorescently-labeled antibody that specifically binds to Bim, a pro-apoptotic Bcl-2 family member.

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4 protocols using bim pe

1

Comprehensive Immune Profiling of PBMC Populations

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The following panel of antibodies was used for analysis of PBMC populations: CD8-PE-Cy7 (BD Pharmingen, clone RPA-T8, catalog 304006), CD11a-APC (BioLegend, clone HI111, catalog 301212), PD-1 FITC (BioLegend, clone EH12.2H7, catalog 32990), CX3CR1-APC/Cy7 (BioLegend, clone 2A9-1, catalog 341616), Bim-PE (Cell Signaling Technology, Danvers, Massachusett, clone C34C5, catalog 12186S), Ki-67-BV421(BD Biosciences, clone B56, catalog 562899), Granzyme B-PerCP (Novus Biologicals, Danvers, Massachusett, clone CLB-GB11, catalog NBP1-50071PCP). CD8+ T-cells were first stained for surface markers followed by intracellular staining. Flow cytometry data were collected on a CytoFLEX LX (Beckman Coulter, Atlanta, Georgia). Flow cytometry analysis was performed with FlowJo software 10.4 (Tree Star, Palo Alto, California).
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2

Immunophenotyping of PBMC Populations

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The following panel of antibodies was used for analysis of PBMC populations: CD8-PE-Cy7 (BD Pharmingen, clone RPA-T8, catalog 304006), CD11a-APC (BioLegend, clone HI111, catalog 301212), PD-1 FITC (BioLegend, clone EH12.2H7, catalog 32990), CX3CR1-APC/Cy7 (BioLegend, clone 2A9–1, catalog 341616), Bim-PE (Cell Signaling Technology, clone C34C5, catalog 12186S), Ki-67-BV421(BD Biosciences, clone B56, catalog 562899), Granzyme B-PerCP (Novus Biologicals, clone CLB-GB11, catalog NBP1–50071PCP). CD8+ T-cells were first stained for surface markers followed by intracellular staining. Flow cytometry data was collected on a CytoFLEX LX (Beckman Coulter). Flow cytometry analysis was performed with FlowJo software 10.4 (Tree Star).
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3

Multiparametric Immune Cell Profiling

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Peripheral Blood Mononuclear Cells values were isolated, and subpopulations were identified with the following antibody panel: CD8-PE-Cy7 (BD Pharmingen, clone RPA-T8, catalog 304006), CD11a-APC (BioLegend, clone HI111, catalog 301212), PD-1 FITC (BioLegend, clone EH12.2H7, catalog 32990), Bim-PE (Cell Signaling Technology, clone CD34C5, catalog 12186S), Granzyme B-PerCP (Novus Biologicals, clone CLB0GB11, catalog NBP1–50071PCP), and CX3CR1-APC/Cy7 (BioLegend, clone 2A9–1, catalog 341616). Flow cytometry was performed on a CytoFLEX LX (Beckman Coulter, Atlanta, Georgia). Data were analyzed with FlowJo, version 10.6.1.
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4

Multiparametric Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Peripheral Blood Mononuclear Cells values were isolated, and subpopulations were identified with the following antibody panel: CD8-PE-Cy7 (BD Pharmingen, clone RPA-T8, catalog 304006), CD11a-APC (BioLegend, clone HI111, catalog 301212), PD-1 FITC (BioLegend, clone EH12.2H7, catalog 32990), Bim-PE (Cell Signaling Technology, clone CD34C5, catalog 12186S), Granzyme B-PerCP (Novus Biologicals, clone CLB0GB11, catalog NBP1–50071PCP), and CX3CR1-APC/Cy7 (BioLegend, clone 2A9–1, catalog 341616). Flow cytometry was performed on a CytoFLEX LX (Beckman Coulter, Atlanta, Georgia). Data were analyzed with FlowJo, version 10.6.1.
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