Trasfectin
TrasFectin is a transfection reagent designed for the efficient delivery of nucleic acids, such as plasmid DNA, siRNA, and mRNA, into a variety of cell types. It facilitates the uptake and expression of the delivered genetic material within the target cells.
Lab products found in correlation
2 protocols using trasfectin
Lentiviral Knockdown of BARD1 Gene
Knocking Down HIF1A and EPAS1 Expression
HEK293T were transfected using 10 μg shRNA plasmid DNA, 30 μl Trans-Lentiviral Packaging Mix (OpenBiosystem), and 25 μl TrasFectin (Bio-Rad), in 10-mm plates. The supernatants (10 ml per condition) were harvested after 24 h, centrifuged at a low speed to remove cell debris, and filtered through 0.45-μm filters. In-vitro transduction and determination of the lentivector titre were performer as reported previously58 (link). After 48 h of incubation, the transduced cells were examined microscopically for the presence of TurboGFP expression (70%–90%). To obtain 100% GFP-positive cells, puromycin was added into the medium for an additional 10 days. The reported data are representative of the experiments performed and confirmed using both lentiviral vectors for each gene.
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