cd47−/− and thbs1−/− mice were purchased from Jackson Laboratories and backcrossed to a pure C57Bl/6 background before use. Wild type C57Bl/6 mice were purchased from Charles River Laboratories under contract from the National Cancer Institute and maintained in the same vivarium for at least one month to ensure similar microflora. Animals were age and sex-matched. All experiments were performed in an accredited facility according to the NIH guidelines under protocols LP-012 and LP-022 approved by the National Cancer Institute Animal Care and Use Committee.
Thbs1 mice
Manufactured by Jackson ImmunoResearch
Thbs1−/− mice are genetically modified mice that have a knockout of the Thbs1 gene, which encodes the protein thrombospondin-1. These mice lack the expression of thrombospondin-1, a multifunctional protein involved in various physiological and pathological processes.
Automatically generated - may contain errors
2 protocols using thbs1 mice
1
Genetic Knockout Mouse Model Analyses
2
Mouse Models for Metabolic Research
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All animal procedures were performed according to protocols approved by the Institutional Animal Care and Use Committee and in agreement with the National Institutes of Health Guide for the Care and Use of Laboratory Animals. Animals were housed in AALAC approved animal facilities of the Cleveland Clinic. Wild type C57BL6, BALB/c, Lepr db/db , Dock7 m +/Lepr db (heterozygote control, lean and normoglycemic), and Thbs1 -/- mice were purchased from The Jackson Laboratories. WT C57BL6 mice were fed a chow or Western diet (TD.88137, 40-45% kcal from fat, 34% sucrose by weight, Envigo) starting at 4 weeks of age.
Animals were sacrificed at end point by exsanguination under anesthesia with ketamine/xylazine (100 mg/15 mg/kg), and organs were collected. Mice used for cell isolation were euthanized by CO 2 asphyxiation followed by the cervical dislocation.
Animals were sacrificed at end point by exsanguination under anesthesia with ketamine/xylazine (100 mg/15 mg/kg), and organs were collected. Mice used for cell isolation were euthanized by CO 2 asphyxiation followed by the cervical dislocation.
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