Gr144053

Manufactured by Bio-Techne

GR144053 is a type of lab equipment manufactured by Bio-Techne. It is a device designed for use in laboratory settings, but its core function is not detailed in this unbiased, factual description.

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Lab products found in correlation

Dasatinib, by Selleck Chemicals (1 mentions) Anti human cd61 antibody, by BD (1 mentions) Alexa fluor 647 conjugated human fibrinogen, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 conjugated annexin 5, by Thermo Fisher Scientific (1 mentions) Phe pro arg chloromethylketone ppack, by Prolytix (1 mentions) Corn trypsin inhibitor cti, by Prolytix (1 mentions) Sigmacote, by Merck Group (1 mentions) H gly pro arg pro oh gprp, by Merck Group (1 mentions) Collagen type 1, by Chrono-Log (1 mentions) Ab197010, by Abcam (1 mentions)

2 protocols using gr144053

Platelet Activation Signaling Assay

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Reagents were obtained as follows: anti-human CD61 antibody (BD Biosciences, San Jose, CA. Cat#: 555754), Alexa Fluor 647–conjugated human fibrinogen (Life Technologies, Grand Island, NY. Cat#: F35200), Alexa Fluor 488 phospho-Src (Tyr418) polyclonal antibody (ThermoFisher Scientific, Waltham, MA. Cat#: 44–660A1), Dade Innovin prothrombin time (PT) reagent (Siemens, Malvern, PA. Cat#: B4212–40), collagen (type I; Chrono-Log, Havertown, PA. Cat#: 385), Sigmacote® (Millipore Sigma, Burlington, MA. Cat#: SL2–100ML), H-Gly-Pro-Arg-Pro-OH (GPRP; Millipore Sigma, Burlington, MA. Cat#: 03-34-0001), dasatinib (Selleckchem, Houston, TX. Cat#: S1021), GS-9973 (entospletinib) (Selleckchem, Houston, TX. Cat#: S7523), Alexa Fluor 488-conjugated annexin V (ThermoFisher Scientific, Waltham, MA. Cat#: A13201), GR144053 (Tocris Biosciences, Bristol, UK. Cat#: 1263), Phe-Pro-Arg-chloromethylketone (PPACK, Haematologic Technologies, Essex Junction, VT. Cat#: FPRCK-01) and corn trypsin inhibitor (CTI, Haematologic Technologies, Essex Junction, VT. Cat#: CTI-01).

Zhang Y, & Diamond S.L. (2020). Src family kinases inhibition by dasatinib blocks initial and subsequent platelet deposition on collagen under flow, but lacks efficacy with thrombin generation.. Thrombosis research, 192, 141-151.

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Neuronal Cell Treatment Protocol

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Tun was acquired as a ready-made solution in DMSO (SML1287-1ML, Merck). IOWH032 (#S7329, Selleckchem) was dissolved in DMSO; GR144053 (#1263, Tocris) and L-Moses (#6251, Tocris) were dissolved in dH₂O as per manufacturer instructions. D14 neurons were treated with 100 nM Tun, unless otherwise stated. Inhibitors were added two days before Tun-treatment onset. For the BRB-seq and Mass spectrometry experiments, 12.5 or 25 μM L-Moses was used for cortical or dopaminergic neurons, respectively.
Viability was measured by MTS assay (#ab197010, Abcam) according to manufacturer’s instructions.

Pavlou S., Foskolou S., Patikas N., Field S.F., Papachristou E.K., Santos C.D., Edwards A.R., Kishore K., Ansari R., Rajan S.S., Fernandes H.J, & Metzakopian E. (2023). CRISPR-Cas9 genetic screen leads to the discovery of L-Moses, a KAT2B inhibitor that attenuates Tunicamycin-mediated neuronal cell death. Scientific Reports, 13, 3934.

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