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Neurotrace dye

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NeuroTrace Dye is a fluorescent dye used for labeling neurons and neural tissue. It can be used to visualize and analyze neuronal structures in a variety of applications, such as cell imaging and histological analysis.

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Lab products found in correlation

Nis elements software, by Nikon (3 mentions) N a plan fluor apochromat objective, by Olympus (2 mentions) 90i upright fluorescence microscope, by Nikon (1 mentions)

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NeuroTrace (62 citations) NeuroTrace 640/660 deep-red fluorescent dye (2 citations)

3 protocols using neurotrace dye

1

Unbiased Stereological Quantification of Nigral Dopaminergic Neurons

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The total number of surviving dopaminergic neurons in the substantia nigra was determined using an adapted protocol from Tapias et al. (2013), employing an unbiased, automated stereological system. Briefly, 1 in every 6th nigral tissue section were stained for TH and counterstained with NeuroTrace Dye (640; Life Technologies). Tissue sections were scanned and imaged using an Olympus microscope equipped with 20× objective high N.A. plan fluor/apochromat objective. Twenty times magnification was used to generate montage imaging of the ventral midbrain, for which the entire substantia nigra was analyzed per image using anatomical region of interest boundaries. Images were processed using Nikon NIS-Elements software, and quantitative analysis was performed on fluorescent images colocalizing TH and Nissl-positive stains.
Rocha E.M., De Miranda B.R., Castro S., Drolet R., Hatcher N.G., Yao L., Smith S.M., Keeney M.T., Di Maio R., Kofler J., Hastings T.G, & Greenamyre J.T. (2019). LRRK2 inhibition prevents endolysosomal deficits seen in human Parkinson’s disease. Neurobiology of disease, 134, 104626.
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2

Quantitative Analysis of Nigral Dopamine Neurons

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Stereological analysis of dopamine neuron number in the SN was achieved using an adapted protocol from Tapias et al. (2013), employing an unbiased, automated system. Briefly, nigral tissue sections were stained for TH and counterstained with DAPI and NeuroTrace Dye (640; Life Technologies) and imaged using a Nikon 90i upright fluorescence microscope equipped with high N.A. plan fluor/apochromat objectives, Renishaw linear encoded microscope stage (Prior Electronics) and Q-imaging Retiga cooled CCD camera (Center for Biological Imaging, University of Pittsburgh). Images were processed using Nikon NIS-Elements software, and quantitative analysis was performed on fluorescent images colocalizing DAPI, TH, and Nissl-positive stains.
De Miranda B.R., Rocha E.M., Bai Q., El Ayadi A., Hinkle D., Burton E.A, & Greenamyre J.T. (2018). Astrocyte-specific DJ-1 overexpression protects against rotenone-induced neurotoxicity in a rat model of Parkinson’s disease. Neurobiology of disease, 115, 101-114.
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3

Unbiased Stereological Quantification of Nigral Dopaminergic Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols
The total number of surviving dopaminergic neurons in the substantia nigra was determined using an adapted protocol from Tapias et al. (2013), employing an unbiased, automated stereological system. Briefly, 1 in every 6th nigral tissue section were stained for TH and counterstained with NeuroTrace Dye (640; Life Technologies). Tissue sections were scanned and imaged using an Olympus microscope equipped with 20× objective high N.A. plan fluor/apochromat objective. Twenty times magnification was used to generate montage imaging of the ventral midbrain, for which the entire substantia nigra was analyzed per image using anatomical region of interest boundaries. Images were processed using Nikon NIS-Elements software, and quantitative analysis was performed on fluorescent images colocalizing TH and Nissl-positive stains.
Rocha E.M., De Miranda B.R., Castro S., Drolet R., Hatcher N.G., Yao L., Smith S.M., Keeney M.T., Di Maio R., Kofler J., Hastings T.G, & Greenamyre J.T. (2019). LRRK2 inhibition prevents endolysosomal deficits seen in human Parkinson’s disease. Neurobiology of disease, 134, 104626.
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