Electric eel acetylcholinesterase (Type VI‐S), acetylthiocholine iodide, equine serum butyrylcholinesterase, S‐butyrylthiocholine iodide, 5,5′‐dithiobis‐(2‐nitrobenzoic acid) (DTNB), neostigmine, 2, 2‐diphenyl‐1‐picrylhydrazyl (DPPH), Folin–Ciocalteu reagent, gallic acid, quercetin, ascorbic acid, ciprofloxacin, Luria‐Bertani (LB) and nutrient agar media were purchased from Sigma‐Aldrich. Ethanol, Na2CO3, KH2PO4, K2HPO4, aluminum chloride, and methanol were provided by Merck.
Nutrient agar media
Manufactured by Merck Group
Sourced in United States
Nutrient agar media is a growth medium used in microbiology laboratories to cultivate a wide range of microorganisms, such as bacteria and fungi. It provides the necessary nutrients and solidifying agent to support the growth and development of these microorganisms under controlled conditions.
Automatically generated - may contain errors
Lab products found in correlation
Gallic acid, by Merck Group (4 mentions)
Quercetin, by Merck Group (3 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (3 mentions)
Methanol, by Merck Group (3 mentions)
Ethanol, by Merck Group (3 mentions)
Ascorbic acid, by Merck Group (2 mentions)
Folin ciocalteu reagent, by Merck Group (2 mentions)
Trichloroacetic acid, by Merck Group (2 mentions)
Potassium ferricyanide, by Merck Group (2 mentions)
Sodium carbonate, by Merck Group (2 mentions)
Neostigmine, by Merck Group (1 mentions)
Equine serum butyrylcholinesterase, by Merck Group (1 mentions)
Acetylthiocholine iodide, by Merck Group (1 mentions)
Luria bertani, by Merck Group (1 mentions)
Electric eel acetylcholinesterase type 6 s, by Merck Group (1 mentions)
S butyrylthiocholine iodide, by Merck Group (1 mentions)
Ciprofloxacin, by Merck Group (1 mentions)
5 5 dithiobis 2 nitrobenzoic acid, by Merck Group (1 mentions)
K2hpo4, by Merck Group (1 mentions)
Kh2po4, by Merck Group (1 mentions)
9 protocols using nutrient agar media
1
Acetylcholinesterase Activity and Antioxidant Assays
2
Bioactive Compounds and Antimicrobial Activity
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Cucumber (Cucumis sativus) and kidney bean (Phaseolus vulgaris) L. seeds were purchased from the local market. Chicken, duck, and quail eggs were obtained from a private farm (Zagazig City, Egypt). Proteins’ isolates were prepared at Biochemistry Department, Faculty of Agriculture, Zagazig University, Egypt. Pepsin enzyme, DPPH, Gallic acid, quercetin, benzoic acid, sodium benzoate, citric acid, sodium citrate, and nutrient agar media (lab-lemco powder) were purchased from Sigma (Ronkonkoma, NY, USA), MacConkey agar (Heywood, UK). The G+ bacteria (Listeria monocytogenes ATCC 15313, Bacillus cereus ATCC 11778, and G− bacteria (E. coli ATCC 25922, and Pseudomonas aeruginosa ATCC 27853) were purified active strains. Fungi strains, Aspergillus niger, Penicillium notatum, and Fusarium solani were obtained from the Cairo MIRCEN, Ain Shams University, Cairo, Egypt.
3
Antimicrobial Nanoparticle Synthesis and Evaluation
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Prepared silver nanoparticles (99.99%, Guangzhou Hongwu Material Technology, Guangzhou, China), graphene nanoparticles (100%, Knano, Xiamen, China), de ionized water, methylene blue and nutrient agar media (99.98%, Sigma Aldrich, Saint Louis, MO, USA) were used as chemical materials in this study.
4
Extraction and Characterization of Palm Sheath Fibers
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Palm (Phoenix dactylifera L.) sheath fibers (PSFs) were collected after pruning in the fall of 2019 from Sohag Governorate in Upper Egypt. All chemicals used in this study were of analytical grade and used as supplied without any further purification. APS and nutrient agar media were obtained from Sigma-Aldrich. Sulfuric acid (97%) and zinc acetate dihydrate were procured from Abco Chemie (ENG. Ltd.) and Laboratory Rasayan, respectively. The DNA extraction of samples was performed using a QIAamp DNA minikit and a 2 × QuantiTect SYBR Green PCR Master Mix (both from Qiagen GmbH, Germany).
5
Isolation of Bacterial Strains from Canola Rhizosphere
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The soil sample was obtained from the rhizosphere of the canola plant growing in the arid region of Bahawalpur (29.3544° N, 71.6911° E), Pakistan. The soil samples were used to make serial dilutions (15 dilutions) by adding 1 g of soil to 9 mL of water. Dilution was prepared by mixing 1 mL suspension with 9 mL distilled water and total of 10 serial dilutions were prepared. Isolation was done by using nutrient agar media (Sigma Aldrich, USA). The Petri plates were incubated at 28 °C ± 2 in an incubator for 48 to 72 h and observed for the appearance of colonies. Bacterial strains were isolated by repeated streaking [31 ].
6
Textile Dye Decolorization using Nutrient Agar
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The textile dyes (azo dye compounds), namely DR 31 and DB 151, were purchased from the textile industry. Nutrient agar media and all other chemicals used in mineral salt medium (MSM) preparation were of analytical grade and purchased from Merck, India.
7
Analytical Evaluation of Bioactive Compounds
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The study involves use of analytical grade solvents i.e. n-hexane; chloroform; acetone; ethyl acetate; ethanol; methanol and dimethyl sulfoxide (DMSO) purchased from Sigma (Sigma-Aldrich, USA). The reagents include gallic acid; quercetin; 2,2-diphenyl-1-picryl-hydrazyl (DPPH); potassium acetate; aluminum chloride; Folin-Ciocalteu (F-C) reagent; sodium carbonate; ascorbic acid; ammonium molybdate; sodium phosphate; sulfuric acid; ferric cyanide; trichloroacetic acid and potassium ferricyanide procured from Merck (Merck KGaA, Germany), rutin; myricetin; caffiec acid;apigenin;kaempferol; catechin; and caffeic acid were purchased from Sigma (Sigma-Aldrich, Germany). Sabouraud dextrose agar (SDA), nutrient broth and nutrient agar media were purchased from Merck (Merck KGaA, Germany). RPMI-1640, fetal bovine serum (FBS) and medium 199 were purchased from Sigma (Sigma Chemical Co., St. Louis, MO). Equipment included Agilent 1200 series binary gradient HPLC coupled with diode array detector (Agilent Technologies, Germany); CO2 incubator (mco-17AIC,Sanyo-Japan); improved neubauer chamber (Marien, Germany). Reference standards employed in the current study, werepurchased from Sigma-Aldrich USA, include droxithromycin; cefixime; terbinafine; doxorubicin; surfactin; amphotericin-B; 5-Florouracil and vincristine.
8
Antimicrobial and Antioxidant Potential of Resveratrol
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Materials were supplied from companies as follow: butylated hydroxytoluene (BHT), calcium chloride, potassium phosphate buffer, potassium ferricyanide, trichloroacetic acid, ferric chloride, ethanol, methanol, sodium carbonate, glycerol, Folin–Ciocalteu reagent, 1 1‐diphenyl‐2‐picrylhydrazyl (DPPH), CMC powder, resveratrol powder, and Gallic acid were purchased from Sigma (Sigma‐Aldrich). Also, brain heart infusion (BHI) agar, BHI broth, and nutrient agar media were purchased from Merck (Merck). Lyophilized vials of Staphylococcus aureus (ATCC 25923), Salmonella enteritidis (ATCC 14028), Listeria monocytogenes (ATCC 13932), and Escherichia coli (ATCC 15224) were bought from microbial collection of the Iranian Research Organization for Science and Technology (IROST).
9
Synthesis of Zinc Acetate Dihydrate
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Zinc acetate dihydrate (Zn(CH3COO)2·2H2O), sodium hydroxide (NaOH),
and ethanol were purchased from Aldrich. Nutrient Agar Media was procured
from Merck. All of the reagents used were of analytical grade. Laboratory
glassware was kept overnight in a 10% (v/v) HNO3 solution
and then rinsed with deionized water.
and ethanol were purchased from Aldrich. Nutrient Agar Media was procured
from Merck. All of the reagents used were of analytical grade. Laboratory
glassware was kept overnight in a 10% (v/v) HNO3 solution
and then rinsed with deionized water.
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