Concentrations of plasma LBP were measured using commercially available enzyme-linked immunosorbent assay (ELISA) kits (Duoset, R&D Systems, Minneapolis, MN, USA) in duplicate according to the manufacturer's instruction. Plasma samples were diluted 1,000 folds. The minimum detectable concentration of LBP was 0.78 ng/mL. No cross-reactivity or interference was observed with LBP. The absorbance values for the ELISA were determined using an Infinite 2000 Pro Multimode Plate Reader (Tecan, Vienna, VA, USA) at an optical absorbance of 450 nm. The final concentration was determined with reference to a standard curve.
Infinite 2000 pro multimode plate reader
Manufactured by Tecan
Sourced in United States
The Infinite 2000 Pro Multimode Plate Reader is a versatile laboratory instrument designed for performing various detection modes in microplate-based assays. It is capable of absorbance, fluorescence, and luminescence measurements.
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Lab products found in correlation
2 protocols using infinite 2000 pro multimode plate reader
1
Plasma LBP Quantification by ELISA
2
Quantifying Serum LBP Levels
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The serum concentration of LBP was measured using a commercially available enzyme-linked immunosorbent assay (ELISA) kit (Duoset, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions and with all measurements in duplicate. Serum samples were diluted 1000-fold before this assay. No significant cross-reactivity or interference with LBP was observed. A450 values with a calibration line obtained with an LBP standard preparation were recorded using an Infinite 2000 Pro Multimode Plate Reader (Tecan, Vienna, VA, USA).
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