Dorsomorphin 2hcl

The two pancreatic cancer cell lines, Kras^G12D (hereafter denoted as 399 and 403) and Kras^G12D-LOH (exhibiting a loss of heterozygosity of Kras^G12D, hereafter denoted as 907 and 897), were generous gifts from the Technical University of Munich. All four cell lines were isolated from transgenic p48^Cre/+; LSL-Kras^G12D/+; Tsc1^fl/+ mice as previously described 26 (link). All cells were incubated in Dulbecco's Modified Eagle's Medium (DMEM, HyClone, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS, HyClone, Logan, UT, USA) and 100 U/ml penicillin and streptomycin (Beyotime Biotechnology Corporation, Shanghai, China) at 37ºC under normoxia (95% air, 5% CO₂) or hypoxia (1% O₂, 94% N₂ and 5% CO₂). For Compound C stimulation, the cells were incubated with Dorsomorphin 2HCl (10 μM, Selleck, Houston, TX, USA) for 24 hours before further measurement, and cells treated with the same volume of the vehicle were used as the control group.

HCT116, HeLa, and PC-3 cells were obtained from American Type Culture Collection (Rockville, MD, USA). Huh7 cells were obtained from Cell Bank of Chinese Academy of Sciences (Shanghai, China). HCT116 cells were cultured in McCoy’s 5A medium. HeLa, PC-3, and Huh7 cells were cultured in Dulbecco’s modified Eagle’s medium. All media contained 10% fetal bovine serum and 10 units/mL penicillin, as well as 10 units/mL streptomycin. For heat stress, the cells were exposed to 42°C in a humidified atmosphere with 5% CO₂. Dorsomorphin, Dorsomorphin·2HCl, MG132, and 17-AAG were obtained from Selleck Chemicals (Houston, TX, USA). Dorsomorphin derivatives were purchased from ChemPartner (Shanghai, China).