Sodium alginate (Na-Alg, viscosity of 1% solution at 20 °C of 300–400 mPa s), barium chloride (BaCl2) dihydrate, and calcium chloride (CaCl2) dihydrate were obtained from Wako Pure Chemical (Osaka, Japan). RGD peptide-coupled alginate (NOVATACH LVM GRGDSP peptide-coupled alginate; RGD-Alg) was obtained from FMC BioPolymer (Sandvika, Norway). Fetal bovine serum (FBS), 1 M HEPES buffer, LIVE/DEAD Viability/Cytotoxicity kit, green fluorescent microbeads (Φ of 1.0 μm and 3.1 μm), Prolong Gold antifade reagent with DAPI, and Alexa Fluor 488 goat anti-rabbit IgG were obtained from Thermo Fisher Scientific (MA, USA). Polydimethylsiloxane (PDMS) prepolymer (Silpot 184) was obtained from Dow Corning Toray (Tokyo, Japan). (Heptadecafluoro-1,1,2,2-tetrahydrodecyl) triethoxysilane (fluorosilane) was obtained from Gelest (PA, USA). Poly-l -lysine (PLL; average Mv of 150 000–300 000), Dulbecco's modified Eagle's medium (DMEM), penicillin–streptomycin solution, trypsin/EDTA solution, PKH26 red fluorescent cell linker kit, PKH67 green fluorescent cell linker kit, and alginate lyase (from Flavobacterium multivorum) were obtained from Sigma Aldrich (MO, USA). Rabbit polyclonal anti-elastin antibody was obtained from Abcam (Cambridge, UK). All other chemicals were of analytical grade.
Green fluorescent microbeads
Manufactured by Thermo Fisher Scientific
Sourced in United States
Green fluorescent microbeads are microscopic particles that emit green fluorescent light when exposed to a specific wavelength of light. These beads are designed for use in various scientific applications, such as flow cytometry, cell labeling, and assay development.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Sodium alginate na alg, by Fujifilm (2 mentions)
Calcium chloride cacl2 dihydrate, by Fujifilm (2 mentions)
Alginate lyase from flavobacterium multivorum, by Merck Group (2 mentions)
Dulbecco s modified eagle s medium dmem, by Merck Group (2 mentions)
Penicillin streptomycin solution, by Merck Group (2 mentions)
Alexa fluor 488 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Live dead viability cytotoxicity kit, by Thermo Fisher Scientific (1 mentions)
Pkh26 red fluorescent cell linker kit, by Merck Group (1 mentions)
Hepes buffer 1 m, by Thermo Fisher Scientific (1 mentions)
Rabbit polyclonal anti elastin antibody, by Abcam (1 mentions)
Pkh67 green fluorescent cell linker kit, by Merck Group (1 mentions)
Prolong gold antifade reagent with dapi, by Thermo Fisher Scientific (1 mentions)
Poly l lysine (pll), by Merck Group (1 mentions)
Trypsin edta solution, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Rockland Immunochemicals (1 mentions)
Gelatin, by Fujifilm (1 mentions)
Hemoglobin from bovine blood, by Merck Group (1 mentions)
Trypsin ethylenediaminetetraacetic acid edta solution, by Merck Group (1 mentions)
Glutaraldehyde solution, by Fujifilm (1 mentions)
2 protocols using green fluorescent microbeads
1
Regenerative Engineered Tissue Constructs
2
Alginate-Gelatin Hydrogel Fabrication
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sodium alginate (Na-alg; viscosity of 300–400
mPa s at 1% solution at 20 °C), gelatin (from bovine bone; pH
of a 50 g L–1 solution at 35 °C of 5.5–6.0,
catalog number of 076-02765), calcium chloride (CaCl2)
dihydrate, 25% glutaraldehyde solution, and trisodium citrate dihydrate
were obtained from Fujifilm Wako Pure Chemical (Osaka, Japan). Dextran
(from Leuconostoc spp., Mr of 450,000–650,000), Dulbecco’s modified
Eagle’s medium (DMEM), penicillin–streptomycin solution,
trypsin/ethylenediaminetetraacetic acid (EDTA) solution, alginate
lyase (from Flavobacterium multivorum),
and hemoglobin (from bovine blood) were obtained from Sigma-Aldrich
(MO, USA). Polydimethylsiloxane (PDMS) prepolymer (Silpot 184) was
obtained from Dow Corning Toray (Tokyo, Japan). Bovine serum albumin
(BSA) was obtained from Rockland Immunochemicals (PA, USA). Phosphate-buffered
saline was obtained from Takara Bio (Shiga, Japan). Fetal bovine serum
(FBS), green fluorescent microbeads (diameter of 0.5 μm), and
alamarBlue cell viability reagent were obtained from Thermo Fisher
Scientific (MA, USA). These materials were used as received. All other
chemicals were of analytical grade.
mPa s at 1% solution at 20 °C), gelatin (from bovine bone; pH
of a 50 g L–1 solution at 35 °C of 5.5–6.0,
catalog number of 076-02765), calcium chloride (CaCl2)
dihydrate, 25% glutaraldehyde solution, and trisodium citrate dihydrate
were obtained from Fujifilm Wako Pure Chemical (Osaka, Japan). Dextran
(from Leuconostoc spp., Mr of 450,000–650,000), Dulbecco’s modified
Eagle’s medium (DMEM), penicillin–streptomycin solution,
trypsin/ethylenediaminetetraacetic acid (EDTA) solution, alginate
lyase (from Flavobacterium multivorum),
and hemoglobin (from bovine blood) were obtained from Sigma-Aldrich
(MO, USA). Polydimethylsiloxane (PDMS) prepolymer (Silpot 184) was
obtained from Dow Corning Toray (Tokyo, Japan). Bovine serum albumin
(BSA) was obtained from Rockland Immunochemicals (PA, USA). Phosphate-buffered
saline was obtained from Takara Bio (Shiga, Japan). Fetal bovine serum
(FBS), green fluorescent microbeads (diameter of 0.5 μm), and
alamarBlue cell viability reagent were obtained from Thermo Fisher
Scientific (MA, USA). These materials were used as received. All other
chemicals were of analytical grade.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!