Synchron clinical system cx4

For biochemical analysis, the serum aliquots were obtained by placed the blood samples in serum tubes at temperature of 24°C for approximately 30 min. After clotting, the blood tubes were centrifuged at 3000 rpm for 10 min using a Himac CR 21 G centrifuge (Hitachi, Tokyo, Japan). Supernatants were separated out and then stored at -20°C for further analysis. Kidney functions were determined by serum activities of UA, urea, crea, and BUN. All biochemical assays were performed using a Synchron CX4 Clinical System (Beckman Coulter, Brea, CA, United States) according to the manufacturer’s protocol (Beijing Leadman Biochemistry Technology Co., Ltd., Beijing, China).

For biochemical analysis, serum aliquots were obtained by placing the blood samples in serum tubes at a temperature of 24 °C for approximately 30 min. After clotting, the blood tubes were centrifuged at 3000 rpm for 10 min using a Himac CR 21 G centrifuge (Hitachi, Tokyo, Japan). Supernatants were removed and stored at −20 °C for further analysis. Serum chemistry was assessed using a Synchron CX4 Clinical System (Beckman Coulter, Brea, CA USA) according to the manufacturer’s protocol (Beijing Leadman Biochemistry Technology Co. Ltd, Beijing, China). The serum activities of ALB, ALP, ALT and AST were used as biochemical markers of hepatic damage.

Plasma glucose was measured at the bedside by the glucose-oxidase technique (Beckman Glucose Analyzer II, Beckman Instruments, Fullerton, CA, USA). Insulin and C-peptide assays were performed by electrochemiluminescence on a COBAS e411 instrument (Roche, Indianapolis, IN, USA). Plasma GLP-1, GIP and glucagon were assayed using a multiplex immunoassay (Biorad Laboratories, Hercules, CA, USA). Plasma FFA were assayed by standard spectrophotometric methods on a Synchron Clinical System CX4 (Beckman Instruments). Plasma amino acids were measured in all AGT subjects with available blood samples (n = 10) using a reverse-phase, high-performance liquid chromatography system (HPLC), as previously described [27 (link)]. For the purposes of this study, arginine, branched chain amino acids (BCAA; i.e., leucine, isoleucine, and valine) and total amino acids data were analyzed. To estimate insulin sensitivity, we calculated the Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) index, the Matsuda index, the Oral Glucose Insulin Sensitivity index (OGIS), and the hepatic insulin resistance index (HIRI) [31 (link),32 (link)].