Mouse il 10

Manufactured by R&D Systems

Sourced in United States

Mouse IL-10 is a recombinant protein that represents the interleukin-10 cytokine from the mouse. Interleukin-10 is an anti-inflammatory cytokine that plays a role in regulating the immune response.

Automatically generated - may contain errors

Lab products found in correlation

Human il 10, by R&D Systems (2 mentions) Mouse tnf α, by R&D Systems (2 mentions) Human ccn1, by R&D Systems (2 mentions) Mouse mip 2 duoset elisa, by R&D Systems (2 mentions) Mouse ccn1 elisa, by MyBioSource (2 mentions) Mouse il 6, by R&D Systems (2 mentions) Microplate reader, by Agilent Technologies (1 mentions) Mouse il 12, by Thermo Fisher Scientific (1 mentions) Recombinant mouse il 6, by R&D Systems (1 mentions) Tetramethyl benzidine tmb solution, by Thermo Fisher Scientific (1 mentions) Mouse il 1β, by R&D Systems (1 mentions) Mouse il 6, by BD (1 mentions) Mouse tnf α, by BD (1 mentions) Mouse il 10, by Thermo Fisher Scientific (1 mentions)

5 protocols using mouse il 10

ELISA-based Cytokine Quantification in Dendritic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Supernatants were collected from stimulated DCs (16 hrs), and cytokine production was measured by ELISA according to standard protocol. Capture and detection antibody pairs for IL-6 (MP5-20F3 and MP5-32C11), IL-10 (JES5-2A5 and SXC-1), and IL-12 (C15.6 and C17.8) were purchased from BD Biosciences. Recombinant mouse IL-6 (R&D), mouse IL-10 (R&D), and mouse IL-12 (Peprotech) were used as standards. Cytokine-antibody complexes were visualized by the addition of Tetramethyl Benzidine (TMB) solution (Life Technologies), and color development was stopped by the addition of TMB stop solution (Life Technologies). Absorbance at 492 nm was measured on a microplate reader (Biotek).

Speck S., Lim J., Shelake S., Matka M., Stoddard J., Farr A., Kuchroo V, & Laouar Y. (2014). TGF-β Signaling Initiated in Dendritic Cells Instructs Suppressive Effects on Th17 Differentiation at the Site of Neuroinflammation. PLoS ONE, 9(7), e102390.

+ Open protocol

+ Expand

Hepatic Lipid and Oxidative Biomarkers Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Serum alanine aminotransferase was measured using a commercial kit according to the manufacturer’s manual (Thermo Fisher Scientific). Hepatic total cholesterol and triglycerides were analyzed using commercial assay kits (Wako USA, Richmond, VA). Tissue and cellular hydrogen peroxide (H₂O₂) and nitric oxide were measured using commercial assay kits (Invitrogen, and BioVision, Milpitas, CA). Lipid peroxidation was determined using an assay kit for MDA (BioAssay Systems, Hayward, CA). GSH and SOD were analyzed using assay kits from Sigma-Aldrich (St Louis, MO). Tissue cytokines were measured using commercial enzyme-linked immunosorbent assay kits (mouse TNF-α and mouse IL6 from BD Biosciences, San Jose, CA; mouse IL1β and mouse IL10 from R&D Systems, Minneapolis, MN) following the manufacturer’s instructions.

Fang Z., Kim H.G., Huang M., Chowdhury K., Li M.O., Liangpunsakul S, & Dong X.C. (2021). Sestrin Proteins Protect Against Lipotoxicity-Induced Oxidative Stress in the Liver via Suppression of C-Jun N-Terminal Kinases. Cellular and Molecular Gastroenterology and Hepatology, 12(3), 921-942.

+ Open protocol

+ Expand

Cytokine and Chemokine Profiling ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human CCN1 (no cross-activity with CCN2), human IL-10, mouse TNF-α, mouse IL-10, mouse IL-6, mouse MIP-2 duoset ELISA were purchased from R&D systems (Minneapolis, MN) and mouse CCN1 ELISA (no cross-activity with CCN2) was purchased from MyBioSource (San Diego, CA). ELISA was performed per manufacturer’s instruction.

Moon H.G., Qin Z., Quan T., Xie L., Dela Cruz C.S, & Jin Y. (2014). Matrix protein CCN1 induced by bacterial DNA and CpG ODN limits lung inflammation and contributes to innate immune homeostasis. Mucosal immunology, 8(2), 243-253.

+ Open protocol

+ Expand

Measuring Inflammatory Cytokines and Enzymes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tumor necrosis factor (TNF)-α (human and mice), IL-6 (human and mice), IL-10 (human and mice), CXCL1, CXCL2, CXCL5 (all except mouse IL-10: R&D Systems, Minneapolis, MN, USA; mouse IL-10: eBioscience, Thermo Fisher Scientific), elastase, and myeloperoxidase (MPO) (Hycult Biotechnology BV, Uden, the Netherlands) were measured via ELISA according to manufacturers’ recommendations.

Saris A., Qin W., van Linge C.C., Reijnders T.D., Florquin S., Burnet M., Strass S., de Vos A.F, & van der Poll T. (2022). The Azithromycin Pro-Drug CSY5669 Boosts Bacterial Killing While Attenuating Lung Inflammation Associated with Pneumonia Caused by Methicillin-Resistant Staphylococcus aureus. Antimicrobial Agents and Chemotherapy, 66(9), e02298-21.

+ Open protocol

+ Expand

Cytokine and Chemokine Profiling ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!