siRNA molecules and NPs were purchased as described in our previous study (10 (link)). The MXD3 siRNA sense strand sequence was 5'-AUGGACUAAAAGGACCCUUTT-3', and the antisense strand was 5'-AAGGUCCUUUAGUCCAUTT-3'. AllStars Negative siRNA (Qiagen, Hilden, Germany) was used as a negative control. Both MXD3 and control siRNAs were tagged with A488 on the 3' end of the antisense strand (Qiagen). SPIO NPs were purchased from OceanNanotech (San Diego, CA). The SPIO NPs had iron oxide cores measuring 15nm, and outer layers composed of amphiphilic polymer and polyethylenimine groups (PEI). Succinimidyl ester with Alexa Fluor 532 (A532) (Thermo Fisher Scientific) was used to label the SPIO NPs. Clinical-grade doxorubicin and vincristine were provided by the UC Davis Pharmacy. Maphosphamide and clinical-grade cisplatin were purchased from Santa Cruz Biotechnology (Dallas, TX) and the UC Davis Pharmacy, respectively.
Spio nps
Manufactured by Thermo Fisher Scientific
SPIO NPs are superparamagnetic iron oxide nanoparticles. They function as a contrast agent for magnetic resonance imaging (MRI) applications.
Automatically generated - may contain errors
2 protocols using spio nps
1
Nanoparticle-Mediated Delivery of siRNA and Chemotherapeutics
2
Nanoparticle-Mediated Delivery of siRNA and Chemotherapeutics
Check if the same lab product or an alternative is used in the 5 most similar protocols
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siRNA molecules and NPs were purchased as described in our previous study (10 (link)). The MXD3 siRNA sense strand sequence was 5'-AUGGACUAAAAGGACCCUUTT-3', and the antisense strand was 5'-AAGGUCCUUUAGUCCAUTT-3'. AllStars Negative siRNA (Qiagen, Hilden, Germany) was used as a negative control. Both MXD3 and control siRNAs were tagged with A488 on the 3' end of the antisense strand (Qiagen). SPIO NPs were purchased from OceanNanotech (San Diego, CA). The SPIO NPs had iron oxide cores measuring 15nm, and outer layers composed of amphiphilic polymer and polyethylenimine groups (PEI). Succinimidyl ester with Alexa Fluor 532 (A532) (Thermo Fisher Scientific) was used to label the SPIO NPs. Clinical-grade doxorubicin and vincristine were provided by the UC Davis Pharmacy. Maphosphamide and clinical-grade cisplatin were purchased from Santa Cruz Biotechnology (Dallas, TX) and the UC Davis Pharmacy, respectively.
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