The content of TBAs in the liver and intestine tissues were determined using TBA kits (No. E003-2-1; Jiancheng Bioengineering Institute, Nanjing, China).
Tba kit
Manufactured by Nanjing Jiancheng
Sourced in China
The TBA Kit is a laboratory equipment designed for the measurement and analysis of a specific chemical compound or parameter. The kit includes all the necessary components and reagents required to perform the analysis. The core function of the TBA Kit is to provide accurate and reliable results for the target analyte.
Automatically generated - may contain errors
Lab products found in correlation
Rnaprep pure tissue kit, by Tiangen Biotech (1 mentions)
Alp kit, by Nanjing Jiancheng (1 mentions)
Fastquant rt kit, by Tiangen Biotech (1 mentions)
Faststart essential dna green master kit, by Roche (1 mentions)
Dual luciferase reporter assay kit, by Promega (1 mentions)
Ast kit, by Nanjing Jiancheng (1 mentions)
Alt kit, by Nanjing Jiancheng (1 mentions)
6 protocols using tba kit
1
Quantifying Bile Acid Levels in Tissues
2
Serum Biomarker Quantification Protocol
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ALT levels, AST activity and total bile acids (TBA) in serum were analyzed using ALT, AST and TBA kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), respectively.
3
Hepatocyte Apoptosis Evaluation in Mice
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The frozen plasma was returned to room temperature, and alanine aminotransferase (ALT) and aspartate aminotransferase (AST), glutathione S-transferase (GST) activity, and total bile acid (TBA) concentration were measured and calculated according to the kit instructions. ALT kit (No.: C009-2), AST kit (No.: C010-2), GST kit (No.: A004), and TBA kit (No.: E003-2-1) were purchased from Nanjing Jiancheng Bioengineering Institute.
The liver tissues of mice were collected, and routine histological sections were stained with hematoxylin and eosin (HE). Then, the pathological sections were photographed under a microscope. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) was used to detect and analyze the apoptosis of hepatocytes. Image-Pro Plus 6.0 software was used to label and calculate the apoptosis rate.
The liver tissues of mice were collected, and routine histological sections were stained with hematoxylin and eosin (HE). Then, the pathological sections were photographed under a microscope. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) was used to detect and analyze the apoptosis of hepatocytes. Image-Pro Plus 6.0 software was used to label and calculate the apoptosis rate.
4
Serum and Bile Biomarkers Analysis
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The contents of alanine aminotransferase (ALT), aspartate amino transferase (AST), alkaline phosphatase (ALP), total bilirubin (TBIL) and total bile acids (TBA) in serum obtained after centrifugation were determined by Chemistry Analyzer (OLYMPUS AU400, Tokyo, Japan). TBAs level in serum were measured by using Kit from Nanjing Jiancheng Bioengineering institute (Nanjing, China) and detected by a Chemistry Analyzer (OLYMPUS AU400, Tokyo, Japan), according to the manufacturer’s instructions. The whole gallbladder was removed and placed in a test tube to collect bile, bile samples were diluted to 40-fold with normal saline, and TBA content was measured with TBA Kit (Nanjing Jiancheng Bioengineering institute, Nanjing, China). The results were the mean value of eight different animals.
5
Liver Function Biomarker Assays
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The collected blood was subjected to alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), and total bile acid (TBA) assays using the Transaminase (GPT) Activity Assay Kit (Solarbio-BC1555), Transaminase (GOT) Assay Kit (Solarbio-BC1565), TBIL Content Assay Kit (Solarbio-BC5180) and TBA Kit (Nanjing Jiancheng-E003), respectively.
6
Hepatoprotective Compounds Screening
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The compounds tested, with a purity of >98%, were purchased from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Additionally, a-naphthylisothiocyanate (ANIT), OCA, and CDCA with a purity of >98% were purchased from Sigma-Aldrich Chemical Co., Ltd. (Darmstadt, Germany). The dual-luciferase reporter assay kit was procured from Promega (Madison, United States), while the ALT Kit, AST Kit, ALP Kit, and TBA Kit were obtained from Nanjing Jiancheng Institute of Biotechnology (Nanjing, China). Furthermore, the RNAprep Pure Tissue Kit and FastQuant RT Kit were purchased from TIANGEN Biotech Co., Ltd. (Beijing, China), and the FastStart Essential DNA Green Master Kit was acquired from Roche.
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