Sc 41113

Manufactured by Santa Cruz Biotechnology

Sc-41113 is a laboratory equipment product from Santa Cruz Biotechnology. It is designed for general laboratory use. The core function of this product is to provide a solution for researchers and scientists in their day-to-day laboratory activities.

Automatically generated - may contain errors

Lab products found in correlation

Sirna reagent system, by Santa Cruz Biotechnology (2 mentions) Sc 37265, by Santa Cruz Biotechnology (2 mentions) Sirnas, by Santa Cruz Biotechnology (1 mentions) Sc 41109, by Santa Cruz Biotechnology (1 mentions) Control sirna sc 36869, by Santa Cruz Biotechnology (1 mentions) Lipofectamine 2000 kit, by Thermo Fisher Scientific (1 mentions) Control sirna, by Thermo Fisher Scientific (1 mentions) Sc 41446, by Santa Cruz Biotechnology (1 mentions) Gapdh sirna, by Thermo Fisher Scientific (1 mentions) Anti gapdh sirna, by Thermo Fisher Scientific (1 mentions)

3 protocols using sc 41113

siRNA Knockdown of Wnt Genes in MSCs

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siRNAs specific for mouse Wnt3a (sc-41109), Wnt5a (sc-41113) and control siRNA (sc-36869) with scrambled sequences were purchased from Santa Cruz. In each transfection, 0.1 to 0.2 μM siRNAs were introduced into MSCs using the Lipofectamine 2000 kit (Invitrogen, USA). The efficiency of transduction was determined by detecting the rate of FITC-positive cells and the mRNA expression of specific genes.

He X., Wang H., Jin T., Xu Y., Mei L, & Yang J. (2016). TLR4 Activation Promotes Bone Marrow MSC Proliferation and Osteogenic Differentiation via Wnt3a and Wnt5a Signaling. PLoS ONE, 11(3), e0149876.

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Gene Silencing Using siRNA Knockdown

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Atg5, Wnt5a and MMP-3 siRNAs for gene silencing were acquired commercially (sc-41446, sc-41113 and sc-37265, respectively; Santa Cruz Biotechnology Inc.) and were transfected into cultured cells using an siRNA reagent system (Santa Cruz Biotechnology Inc.) according to the manufacturer’s protocol. GAPDH siRNA and control siRNA (Thermo Scientific, Lafayette, CO, USA) with no known homogeny for any vertebrate sequence were used as positive and negative controls, respectively.

Ozeki N., Hase N., Hiyama T., Yamaguchi H., Kawai R., Kondo A., Matsumoto T., Nakata K, & Mogi M. (2015). Interleukin-1β-Induced Autophagy-Related Gene 5 Regulates Proliferation of Embryonic Stem Cell-Derived Odontoblastic Cells. PLoS ONE, 10(4), e0124542.

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Wnt5a, Wnt5b and MMP-3 Silencing Protocol

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The Wnt5a, Wnt5b and MMP-3 siRNAs for gene silencing were acquired commercially (sc-41113, sc-155357 and sc-37265, respectively; Santa Cruz Biotechnology Inc.) and transfected into cultured cells using a siRNA reagent system (Santa Cruz Biotechnology Inc.) according to the manufacturer's protocol. An anti-GAPDH siRNA and an anti-control siRNA, with no known homogeny for any vertebrate sequence (Thermo Scientific, Lafayette, CO, USA), were used as positive and negative controls, respectively.

Hase N., Ozeki N., Hiyama T., Yamaguchi H., Kawai R., Kondo A., Nakata K, & Mogi M. (2015). Products of dentin matrix protein-1 degradation by interleukin-1β-induced matrix metalloproteinase-3 promote proliferation of odontoblastic cells. Bioscience trends, 9(4).

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