Bullfrog adipose tissue was kindly provided by the Asmarana Produtos Naturais (Natal, RN, Brazil). Span® 80 (sorbitan monooleate 80), penicillin-streptomycin, and MTT reagent ((3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide)) were purchased from Sigma Aldrich Inc. (St. Louis, MO, USA). Ethanol, dimethylsulfoxide (DMSO), n-hexane, propylene glycol, and Tween® 20 (polyoxyethylene 20 sorbitan monolaurate) were acquired from VETEC (Rio de Janeiro, RJ, Brazil). Cetostearyl alcohol ethoxylate and isopropyl palmitate were purchased from ViaFarma (São Paulo, SP, Brazil). Xanthan Gum and Germall® were purchased from Mapric (São Paulo, SP, Brazil). Butylhydroxytoluene was acquired from Galena (Campinas, SP, Brazil). Floral green fragrance was obtained from Bio Inter (São Paulo, SP, Brazil).
Dulbecco’s Modified Eagle Medium (DMEM) and fetal bovine serum (FBS) were purchased from Cultilab (Campinas, SP, Brazil). The 3T3 fibroblasts and B16F10 cell lines from the American Type Culture Collection (ATCC) used in this study were provided by the Laboratory of Biotechnology of Natural Polymers at Federal University of Rio Grande do Norte (Natal, RN, Brazil).
Dulbecco’s Modified Eagle Medium (DMEM) and fetal bovine serum (FBS) were purchased from Cultilab (Campinas, SP, Brazil). The 3T3 fibroblasts and B16F10 cell lines from the American Type Culture Collection (ATCC) used in this study were provided by the Laboratory of Biotechnology of Natural Polymers at Federal University of Rio Grande do Norte (Natal, RN, Brazil).