Rabbit monoclonal anti phospho stat1

Cells were lysed in RIPA buffer, and protein concentrations were determined by a BCA Protein Assay Kit (Jiangsu Cowin Biotech, China). A total of 30–50 µg of protein was separated via sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroblotted onto polyvinylidene fluoride membranes (Millipore, Billerica, Massachusetts, USA). Immunoreactivity was determined by using an imaging system (Bio-Rad, USA).
Antibodies specific for the following proteins were used: rabbit monoclonal anti-phospho-STAT1 (1:1,000; Cell Signaling Technology, USA), rabbit monoclonal anti-IRF1 (1:1,000; Proteintech, USA), mouse monoclonal anti-LPCAT3 (1:1,000; Proteintech), anti-β-tubulin (1:5,000; Proteintech) and anti-GAPDH (1:5,000; Proteintech).

Cells were lysed in RIPA buffer, and protein concentrations were determined by a BCA Protein Assay Kit (Jiangsu Cowin Biotech, China). A total of 30–50 µg of protein was separated via sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroblotted onto polyvinylidene fluoride membranes (Millipore, Billerica, Massachusetts, USA). Immunoreactivity was determined by using an imaging system (Bio-Rad, USA).
Antibodies specific for the following proteins were used: rabbit monoclonal anti-phospho-STAT1 (1:1,000; Cell Signaling Technology, USA), rabbit monoclonal anti-IRF1 (1:1,000; Proteintech, USA), mouse monoclonal anti-LPCAT3 (1:1,000; Proteintech), anti-β-tubulin (1:5,000; Proteintech) and anti-GAPDH (1:5,000; Proteintech).