The largest database of trusted experimental protocols

2 protocols using isoprenaline hydrochloride iso

1

Mitochondrial Dynamics Regulation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The phalloidin, JC-1 and DCFH-DA probes were bought from Life Technology (St. Louis, MO, USA). PX-12, DAPI and isoprenaline hydrochloride (Iso) were purchased from Sigma-Aldrich (USA). Rhodamine-conjugated secondary antibodies were obtained from Jackson ImmunoResearch Laboratories (West Grove, PA, USA). STVNa was obtained from Chemical Development Laboratories of Key Biological Pharmaceutical Company (Dongguan, China). Trizol was obtained from Generay (Shanghai, China). Antibodies against Drp1, Trx1, Prdx2 and GAPDH were acquired from Cell Signaling Technology (Beverly, MA, USA). HDAC4 was obtained from Abcam (Cambridge, MA, UK). The Fis1 antibody and HRP-conjugated secondary antibodies were acquired from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
+ Open protocol
+ Expand
2

Signaling Pathway Analysis of Celecoxib

Check if the same lab product or an alternative is used in the 5 most similar protocols
DM-celecoxib was synthesized as described previously [5, 15] . Isoprenaline hydrochloride (ISO) and SB216763 were purchased from Sigma-Aldrich (St. Louis, MO, USA). PTEN inhibitor VO-OHpic trihydrate was from Chem Scene (Monmouth Junction, NJ, USA). Monoclonal anti-GSK-3β and anti-β-catenin antibodies were purchased from BD Biosciences (San Jose, CA, USA). Monoclonal anti-Akt, polyclonal anti-phospho-Akt (Ser473), polyclonal anti-GSK-3α, polyclonal antiphospho-GSK-3α (Ser21), polyclonal anti-phospho-GSK-3β (Ser9), polyclonal anti-mammalian target of rapamycin (mTOR), polyclonal anti-phospho-mTOR (Ser2481), polyclonal anti-matrix metalloproteinasr-2 (MMP-2), and polyclonal anti-phosphatase and tensin homolog on chromosome 10 (PTEN) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Polyclonal anti-fibronectin antibody was from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody was purchased from Abcam (Cambridge, UK).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!