Ge discovery ste pet scanner

[18F]Fallypride, (S)-N-[(1-allyl-2-pyrrolidinyl)methyl]-5-(3[18F]fluoropropyl)-2,3-dimethoxybenzamide was produced in the radiochemistry laboratory attached to the PET unit at Vanderbilt University Medical Center, following synthesis and quality control procedures described by U.S. Food and Drug Administration IND 47,245. PET data were collected on a GE Discovery STE PET scanner (GE Healthcare). Serial scan acquisition was started simultaneously with a 5.0 mCi (185 MBq; Study 1 median specific activity = 5.33 mCi, SD = 0.111; Study 2 median specific activity = 5.32, SD = 0.264) slow bolus injection of DA D2/3 tracer [18F]fallypride (specific activity >3000 Ci/mmol). CT scans were collected for attenuation correction before each of the three emission scans, which together lasted ∼3.5 h with two breaks for subject comfort. Before the PET scan, T1-weighted magnetic resonance (MR) images (Turbo Field Echo [TFE] SENSE protocol; Act. TR = 8.9 ms, TE = 4.6 ms, 192 TFE shots, TFE duration = 1201.9 s, FOV = 256 × 256 mm, voxel size = 1 × 1 × 1 mm) were acquired on a 3T Intera Achieva whole-body scanner (Philips Healthcare).

[¹⁸F]-FE-PE2I was produced by the Vanderbilt Radiochemistry Core laboratory under cGMP protocols. The synthetic preparation was carried out utilizing a Tracerlab FXFN reaction module (GE Healthcare) and was synonymous to a previously published preparation^{50 (link)}. Serial scan acquisition was started simultaneously with a slow bolus injection (mean = 4.63 ± 0.34 mCi; 171.31 ± 12.58 MBq) of the DAT tracer [¹⁸F]-FE-PE2I (specific activity = 2302 ± 1445 Ci/mmol). CT scans were collected for attenuation correction prior to the emission scan. FE-PE2I data were collected during one 61-min dynamic scan with acquisition times listed in Table S1. While previous human PET studies using FE-PE2I used 90 min dynamic scan acquisitions^{46 (link),48 (link)}, we chose the 61 min acquisition time to improve participant comfort given that the correlation between putamen FE-PE2I BP_ND collected after 61 and 90 min in 4 pilot subjects was extremely high (r = 0.997, mean percentage of BP_ND difference = 2.06 ± 1.72). All PET data were collected on a GE Discovery STE PET scanner (GE Healthcare, Milwaukee, USA).