C57BL/6 mice were purchased from the Jackson Laboratory and housed for two weeks prior to infection or spleen harvest. RIP-GP mice, which express the viral GP under the control of the rat insulin promoter, were bred in house [30 (link)]. Smarta mice, TCR transgenic for the dominant I-Ab-restricted LCMV GP61–80 peptide, and FoxP3 GFP reporter mice were bred in house and previously described [33 (link),34 (link)]. Smarta mice were backcrossed onto FoxP3-GFP reporter background and screened for both reporter and TCR transgene expression prior to use. Blood glucose was measured weekly using the OneTouch Ultra system (LifeScan). Mice with blood glucose values >300 mg/dL were scored diabetic.
Onetouch ultra system
Manufactured by LifeScan
The OneTouch Ultra system is a laboratory device designed for the measurement of blood glucose levels. It provides quantitative results through the analysis of a small blood sample.
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Lab products found in correlation
2 protocols using onetouch ultra system
1
Murine Models for LCMV Infection
2
Glucose Tolerance Test in Dlk1 Knockout Mice
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A Glucose Tolerance Test (GTT) was performed in Dlk1−/− and Dlk1+/+ males/females fed HFD or NC. Briefly, animals were fasted for 6 h [33 (link)], and following an intraperitoneal (i.p.) injection of d -Glucose (2 g/kg body weight), blood glucose levels were monitored at indicated time points using the One Touch Ultra® system (LifeScan). In an immunoneutralization experiment, Dlk1+/+ animals (n = 15) fed HFD for 13 weeks were injected i.p. with a mouse monoclonal antibody (IgG1, k, in house) recognizing an epitope on the extracellular part of the mouse DLK1 protein, or with an isotype-matched control antibody (10 μg MAb/g body weight). GTT was performed one and three days after injection.
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