Diode array spectrophotometer

Manufactured by Agilent Technologies

Sourced in United States

The Diode array spectrophotometer is a laboratory instrument used for the measurement and analysis of the absorption spectra of samples. It employs an array of photodiodes to simultaneously detect light intensity at multiple wavelengths, providing a rapid and accurate assessment of the sample's spectral properties.

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Lab products found in correlation

Dialysis tube, by Thermo Fisher Scientific (2 mentions) Origin 8, by OriginLab (1 mentions) Nano zs90, by Malvern Panalytical (1 mentions) 240 elemental analyzer, by PerkinElmer (1 mentions) Xevo g2 qtof, by Waters Corporation (1 mentions) 7000 spectrofluorimeter, by Hitachi (1 mentions) 300 mhz nmr spectrometer, by Bruker (1 mentions) Modulus 2 microplate multimode reader, by Promega (1 mentions) Spectramax m5 spectrometer, by Molecular Devices (1 mentions) Phosphate buffer, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

8453 diode array spectrophotometer (72 citations) Agilent 8453 diode array spectrophotometer (17 citations) 8453 diode-array UV-Vis spectrophotometer (12 citations) HP8452 diode array spectrophotometer (5 citations) Agilent 8453 UV/Vis diode array spectrophotometer (5 citations) Cary 8454 Diode Array System spectrophotometer (3 citations) Model 8453 diode array spectrophotometer (3 citations) Cary 8454 diode array spectrophotometer (2 citations) 8454 UV-VIS Diode Array spectrophotometer (2 citations) 1100 diode array UV/Vis spectrophotometer (2 citations)

7 protocols using diode array spectrophotometer

Steady-state Kinetics of Bacterial Antibiotic Resistance Enzymes

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Steady-state reactions were followed with purified enzymes (KPC-2, OXA-23, OXA-24, PER-2, PDC-3, NDM-1, VIM-2, and IMP-1) using an Agilent diode array spectrophotometer (model 8453) as previously described.^{31 (link)} Assays were performed at 25 °C (room temperature) using either 10 mM PBS, pH 7.4 (KPC-2, PER-2, and PDC-3), 50 mM sodium phosphate buffer supplemented with 20 mM sodium bicarbonate (OXA-23, OXA-24), or 10 mM HEPES, pH 7.5, 0.2 M NaCl, 50 μg/mL bovine serum albumin, and 50 μM Zn (NDM-1, VIM-2, and IMP-1).
Compound 1 was used as a substrate at excess molar concentrations to establish pseudo-first-order kinetics and imipenem (IMI) was used as a control. The following extinction coefficients were used: 1, Δε336 = −5500 M⁻¹ cm⁻¹; IMI, Δε300 = −9000 M⁻¹ cm⁻¹. For velocity determinations, a 1 cm path length quartz cuvette was employed. A nonlinear least square fit of the data (Henri–Michaelis–Menten equation) using Origin 8.1 (OriginLab, Northampton, MA) was employed to obtain the steady-state kinetic parameters V_max, k_cat, and K_m according to eqs 1 and 2

v = (V_{\max} \times [S]) / K_{m} + [S])

k_{cat} = V_{\max} / [E]

Goldberg J.A., Nguyen H., Kumar V., Spencer E.J., Hoyer D., Marshall E.K., Cmolik A., O’Shea M., Marshall S.H., Hujer A.M., Hujer K.M., Rudin S.D., Domitrovic T.N., Bethel C.R., Papp-Wallace K.M., Logan L.K., Perez F., Jacobs M.R., van Duin D., Kreiswirth B.M., Bonomo R.A., Plummer M.S, & van den Akker F. (2020). A γ-Lactam Siderophore Antibiotic Effective against Multidrug-Resistant Gram-Negative Bacilli. Journal of medicinal chemistry, 63(11), 5990-6002.

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Analytical Characterization of DFP-AMQ Complex

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PerkinElmer 240 elemental analyzer was used for elemental analysis. Infrared spectra (400–4000 cm⁻¹) were recorded using KBr pellets on a Nicolet Magna IR 750 series-II FTIR spectrophotometer. A Bruker 300 MHz NMR spectrometer was used for ¹H-NMR recorded in the DMSO-d₆ solvent and using tetramethylsilane (δ = 0) as an internal standard. An Agilent diode-array spectrophotometer (model, Agilent 8453) was used for recording UV-Vis spectra, a Hitachi-7000 spectrofluorimeter was used for recording steady-state fluorescence, ESI-MS⁺ (m/z) of the ligand and Al(iii)-complex were recorded on a Waters' HRMS spectrometer (model: XEVOG2 QTOF). To analyse the morphology of DFP–AMQ in the solid state, FESEM images were taken using an EVO LS10 scanning electron microscope (SEM). For the pH study, a systronics digital pH meter (model 335) was used and 50 mM HCl or NaOH solution was used for the adjustment of pH. The particle size distribution was measured by dynamic light scattering (DLS) on a Malvern Zetasizer Nano ZS90 instrument. Optical fluorescence microscopy images were taken using a LEICA DM1000 LED upright microscope.

Bhowmick R., Mondal P, & Chattopadhyay P. (2023). A new fluorescent probe for sensing Al3+ ions in the solution phase and CH3COO− in the solid state with aggregation induced emission (AIE) activity. RSC Advances, 13(5), 3394-3401.

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Antioxidative Properties of Carbon Quantum Dots

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To perform antioxidative properties study of CQDs a standard procedure with DPPH reagent was perfomed. A 25 mg/L solution of DPPH in 95% ethanol was prepared and used for all tests. To achieve a reliable results the prepared solutions of CQDs were dried overnight and weight of CQDs in solutions was determined. Measure of 0.2 mg/mL of all CQDs were prepared in twice-distilled water. To measure antioxidative properties 2 mL of DPPH solution was mixed with 1 mL of each CQDs solution respectively. The mixtures were keep in closed vials in darkness and the absorbance of blank and test solution was measured at 517 nm by using Agilent diode-array spectrophotometer. The antioxidative properties were calculated by comparision of the absorbance of blank and tested solutuions.

Janus Ł., Piątkowski M, & Radwan-Pragłowska J. (2019). Microwave-Assisted Synthesis and Characterization of Poly(L-lysine)-Based Polymer/Carbon Quantum Dot Nanomaterials for Biomedical Purposes. Materials, 12(23), 3825.

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Spectrophotometric Analysis of RAE and Resorufin

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The ultraviolet–visible spectra of RAE and resorufin solutions were acquired using a diode array spectrophotometer (Agilent Technologies, Santa Clara, CA) in a quartz cuvette. Other absorbance measurements were recorded in either a 96-well plate using a Modulus II Microplate Multimode reader (Promega, Madison, WI) measuring absorbance at 560 nm or in a clear, round bottom 96-well plates on a Spectra Max M5 spectrometer (Molecular Devices, Sunnyvale, CA) under the control of a Windows-based PC running software pro V5. The samples were analysed at λ=580 nm for the resorufin, and at λ=525 nm for RAE.

Koide K., Tracey M.P., Bu X., Jo J., Williams M.J, & Welch C.J. (2016). A competitive and reversible deactivation approach to catalysis-based quantitative assays. Nature Communications, 7, 10691.

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Camelina/Sophia Protein Hydrolysates Inhibit Copper-Induced LDL Oxidation

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The inhibitory activity of camelina/sophia protein hydrolysates against copper-induced LDL cholesterol oxidation was measured according to the reported method [27 (link)]. LDL (5 mg/mL) was dialyzed in 10 mM phosphate buffer (pH 7.4, 0.15 M NaCl) using a dialysis tube with a molecular weight cut-off of 12−14 kDa (Fisher Scientific, Nepean, ON, Canada) at 4 °C under a nitrogen blanket in the dark for 12 h. Diluted LDL cholesterol (0.04 mg LDL/mL) was mixed with protein hydrolysate solutions (0.1 mg/mL). The positive control used was carnosine. The reaction was initiated by adding 0.1 mL of 100 μM solution of CuSO₄. A blank containing only a sample without LDL or CuSO₄ was prepared for each test compound. After incubation of the reaction mixture at 37 ℃ for 12 h, the conjugated dienes formed were recorded at 234 nm using a diode array spectrophotometer (Agilent, Palo Alto, CA, USA). The inhibitory effect of tested samples was expressed as percentage inhibition of conjugated diene formation according to the following equation:

A₀, A_t, and A°₀, A°_t are absorbance values for test samples and control, respectively, measured at zero time and at time t after incubation.

Ngo N.T., Senadheera T.R, & Shahidi F. (2023). Antioxidant Properties and Prediction of Bioactive Peptides Produced from Flixweed (sophia, Descurainis sophia L.) and Camelina (Camelina sativa (L.) Crantz) Seed Meal: Integrated In Vitro and In Silico Studies. Plants, 12(20), 3575.

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Inhibitory Effect of EGC Esters on LDL Peroxidation

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The inhibitory effect of EGC esters on cupric ion-induced human low-density lipoprotein (LDL) peroxidation was determined according to the method described elsewhere [8 ]. Initially, LDL (5 mg/mL) was dialyzed against 100 volumes of freshly prepared phosphate buffer (10 mM, pH 7.4, 0.15M NaCl). A dialysis tube (MWCO of 12–14 kDa, Fischer, Carle and Kammerer Scientific, Nepean, ON, Canada) was used to dialyze LDL at 4 °C under a nitrogen blanket in the dark for 12 h. Diluted LDL cholesterol (0.04 mg LDL/mL) was mixed with the EGC extracts dissolved in ethanol (5 μg/mL, 10 μL) in an Eppendorf tube. The samples were pre-incubated at 37 °C for 15 min and the reaction was initiated by adding a solution of cupric sulphate (0.1 mL, 100 μM). The samples were then incubated at 37 °C for 22 h. The formation of conjugated dienes (CD) was recorded at 234 nm using a diode array spectrophotometer (Agilent, Palo Alto, CA, USA). The appropriate blanks were run for each sample by replacing LDL cholesterol and CuSO₄ and with distilled water for background correction.

Ambigaipalan P., Oh W.Y, & Shahidi F. (2020). Lipophilized epigallocatechin (EGC) and its derivatives: Inhibition of oxidation of β-carotene–linoleate oil-in-water emulsion and DNA strand scission. Journal of Food and Drug Analysis, 28(3), 356-364.

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Lentil Phenolics Inhibit LDL Oxidation

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The inhibitory activity of human LDL peroxidation induced by cupric ion was evaluated according to the method described by Alshikh et al. (2015) . In order to remove EDTA dissolved in LDL cholesterol, LDL was dialyzed using 10 mM phosphate buffer (pH 7.4, 0.15 M NaCl) at 4 °C under a nitrogen blanket in the dark for 12 h. For assessing inhibitory activity against LDL oxidation, 0.8 mL of LDL cholesterol (0.04 mg LDL/mL) was mixed with 0.1 mL of soluble-and insoluble-bound phenolics in hulls and dehulled grains of lentils and subsequently pre-incubated at 37 °C for 15 min. After that, 0.1mL of cupric sulphate (50 μM) was added to the mixture and incubated at 37 °C for 11 h. The conjugated dienes (CD) from the oxidation of LDL cholesterol was read at 234 nm using a diode array spectrophotometer (Agilent, Palo Alto, CA, USA).

Yeo J., & Shahidi F. (2020). Suppressing the oxidation of LDL and DNA strand breakage of bioactives in dehulled and hull fraction of lentils. Journal of Food Bioactives, 12.

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