After harvesting the root nodules from the legumes, nodules were cut longitudinally with a razor blade and embedded in 4% agarose. After polymerization under vacuum conditions for 15min at room temperature, thin sections of 80–90μm were prepared with a Leica VT1000S Microtome. Sections were incubated in 100-fold diluted SYBR® green I nucleic acid stain (Sigma-Aldrich, Darmstadt, Germany) for at least 30min at 4°C. Morphology of the root nodules was investigated with an Olympus SZX12 stereo microscope equipped with Olympus DF PLAPO 1XPF objective lens (Olympus, Shinjuku, Tokio, Japan) coupled with a Zeiss Axiocam 503 color camera (Zeiss, Oberkochen, Germany).
Sybr green 1 nucleic acid stain
Manufactured by Merck Group
Sourced in Germany
SYBR® green I nucleic acid stain is a fluorescent dye used to detect and quantify DNA and RNA. It binds to the minor groove of nucleic acids, resulting in a strong fluorescent signal when excited by light. The dye is commonly used in various applications such as real-time PCR, gel electrophoresis, and nucleic acid quantification.
Automatically generated - may contain errors
Lab products found in correlation
Vt 1000s microtome, by Leica (1 mentions)
Axiocam 503, by Zeiss (1 mentions)
Szx12, by Olympus (1 mentions)
Qubit reagent, by Thermo Fisher Scientific (1 mentions)
Tape station reagents, by Agilent Technologies (1 mentions)
Dneasy plant mini kit, by Qiagen (1 mentions)
Ampure xp bead, by Beckman Coulter (1 mentions)
Quantifluor dsdna system, by Promega (1 mentions)
Ethanol, by Carl Roth (1 mentions)
2 protocols using sybr green 1 nucleic acid stain
1
Legume Root Nodule Microscopic Analysis
2
Plant Genomic DNA Extraction and Sequencing
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Gibberellic acid 4 + 7 (Duchefa Biochemie)
Ethanol (Carl Roth)
Paper bags for bagging plants (HERA)
Bird protection mesh (mesh size 25 mm) (Zill GmbH Co. KG)
Insect protection mesh (mesh size 0.6 mm) (Grow it)
2 ml and 1.5 ml tubes
96 well PCR plates (Sarstedt)
Foil/lids to seal plates
384 Well Lightcycler plates (Sarstedt)
Adhesive Optical film (Biozym Scientific)
Nuclease-free water
Liquid nitrogen
Quiagen DNeasy Plant Mini Kit (Quiagen)
AMPure XP beads (Beckmann Coulter)
Magnetic stand (Applied Biosystems)
KAPA HiFi Hotstart PCR Kit with dNTPs (Roche)
DMSO (Carl Roth)
ROX solution (ThermoFisher Scientific)
SYBR Green I Nucleic acid stain (Sigma-Aldrich)
TE buffer (10 mM Tris–HCl, pH 7.5)
QuantiFluor dsDNA System (Promega)
Tapestation reagents (Agilent Technologies)
Qubit reagents (ThermoFisher Scientific)
NextSeq Reagent Kit (Illumina)
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