Vcam 1

Antibodies against PKC-α, JNK, VCAM-1, CD11b, and β-actin were purchased from GeneTex (Hsinchu, Taiwan). Antibodies against p-PKCα and p-JNK were purchased from Cell Signaling Technology (Danvers, MA, USA). 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein tetrakis(acetoxymethyl) ester (BCECF-AM) and GF109203X, Gö6976 and SP600125 inhibitors were obtained from Sigma-Aldrich (St. Louis, MO, USA).

We purchased p-PLCβ, PLCβ, p-PKCα, PKCα, p-c-Src, c-Src, p-p65, p65, CXCL13, VCAM-1, p-IKK, IKK, p-IκBα, IκBα, and actin from GeneTex International Corporation (Hsinchu City, Taiwan). All ON-TARGETplus siRNAs were purchased from Dharmacon (Lafayette, CO, USA). CXCR5 mAb was purchased from R&D Systems (Minneapolis, MN, USA). Cell culture supplements were purchased from Invitrogen (Carlsbad, CA, USA). A Dual-Luciferase^® Reporter Assay System was bought from Promega (Madison, WI, USA). qPCR primers and probes, as well as the Taqman^® One-Step PCR Master Mix, were supplied by Applied Biosystems (Foster City, CA, USA). The PLCβ inhibitor (U73122), PKCα inhibitor (GF109203X), c-Src inhibitor (PP2), IκB inhibitor (TPCK), NF-κB inhibitor (PDTC), NF-κB inhibitor (BAY 11-7082), and other chemicals not already mentioned were supplied by Sigma-Aldrich (St. Louis, MO, USA).

Anti‐mouse and anti‐rabbit IgG‐conjugated horseradish peroxidase, rabbit polyclonal antibodies specific for PLCβ3 (Santa Cruz, sc‐403), PKCα(Santa Cruz, sc‐208), c‐Src (cell signaling, #2109s), p65 (Santa Cruz, sc‐109), IκBα (Santa Cruz, sc‐1643), p‐PLCβ (cell signaling, #2481s), p‐PKCα(cell signaling, #9375), p‐c‐Src (cell signaling, #5473), p‐p65 (cell signaling, #3033), p‐IKKα/β (cell signaling, #2694), IKKα/β (Santa Cruz, sc‐7218), p‐IκBα (cell signaling, #9246), CXCL13 (GeneTex, GTX108471), CXCR5 (GeneTex, GTX100351), VCAM‐1 (GeneTex, GTX110684) and β‐Actin (GeneTex, GTX109639) were purchased from Cell Signaling Technology, Inc, Santa Cruz Biotechnology, Inc and GeneTex Inc. All other chemicals were obtained from Sigma‐Aldrich. The CXCL13 shRNA plasmid was purchase from RNAiCore (Clone ID: TRCN0000057983). All siRNAs were purchased from Sigma‐Aldrich (Mission^® siRNA).