VEGF-A content of culture medium was quantitated by using Mouse VEGF-A ELISA kit (R&D Systems). 450 nm wavelength of the reaction solution was measured by iMARK microplate leader (BioRad).
Imark microplate leader
Manufactured by Bio-Rad
Sourced in United States
The IMARK microplate reader is a versatile laboratory instrument designed for the detection and analysis of samples in microplate format. It is capable of performing various photometric measurements, including absorbance, fluorescence, and luminescence, across a wide range of wavelengths. The IMARK provides accurate and reliable data for a variety of applications in the fields of biochemistry, cell biology, and molecular biology.
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Lab products found in correlation
2 protocols using imark microplate leader
1
VEGF-A Quantification in Cell Culture
2
MTT Assay for Cell Viability
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Cell survival rate was measured by MTT assay as previously reported [23] (link). After the indicated treatment with drugs was completed, culture medium was replaced with 200 µl fresh medium containing 40 µl MTT solution (2.5 mg/ml, diluted in PBS) and cells were incubated at 37°C for 1.5–2.5 hours. Then, 200 µl lysis buffer containing isopropyl alcohol was applied to each well and mixed by pipetting. Each sample was moved to a 96-well plate and its absorbance at 570 nm was measured using an iMark Micro plate leader (Bio-Rad Laboratories Inc., CA, USA). Cell survival rate was quantitated by absorbance measurement, because MTT (yellow) is deoxidized to formazan (violet) in proportion to mitochondrial activity.
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