Ecat 100

Manufactured by BioAssay Systems

Sourced in United Kingdom, United States

The ECAT-100 is a compact and versatile enzyme-coupled assay system designed for rapid and efficient detection of various analytes. It utilizes a colorimetric detection method to quantify the presence and concentration of the target substance. The ECAT-100 is suitable for a wide range of applications in research and diagnostic laboratories.

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Lab products found in correlation

Rs505, by Randox (1 mentions) Sd125, by Randox (1 mentions) Milliplex rat cytokine immunoassay kit, by Merck Group (1 mentions) Dtac 100, by BioAssay Systems (1 mentions)

2 protocols using ecat 100

Antioxidant Enzyme Assay in Liver Tissue

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The liver tissue samples were homogenized in phosphate-buffered saline (PBS) buffer and then centrifuged for 15 min at 15,000× g to obtain the liver homogenates. The levels of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities were measured using commercial kits (Ransod, SD125, Randox, Crumlin, Antrim, U.K.; ECAT-100, BioAssay Systems, Hayward, CA, USA; RANSEL, RS505, Randox, Crumlin, Antrim, U.K.). The level of glutathione was determined using a EnzyChrom^TM GSH/GSSG assay kit (EGTT-100, BioAssay Systems, Hayward, CA, USA).

Lin T.A., Ke B.J., Cheng C.S., Wang J.J., Wei B.L, & Lee C.L. (2019). Red Quinoa Bran Extracts Protects against Carbon Tetrachloride-Induced Liver Injury and Fibrosis in Mice via Activation of Antioxidative Enzyme Systems and Blocking TGF-β1 Pathway. Nutrients, 11(2), 395.

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Comprehensive Metabolic Biomarker Profiling

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Plasma adiponectin (Millipore, USA. Cat # RAB1136-1KT) and insulin (Millipore, USA. Cat # RAB0904-1KT) were assessed by sandwich ELISA following the manufacturer's protocol. Enzyme-based commercially available assay kits (Biosystems S.A., Spain) were used to measure total cholesterol (Cat # 11,505), triglycerides (Cat # 11,528) and HDL-cholesterol (Cat # 11,648) in circulation following the instructions provided by the manufacturer. Stress parameters such as TAC and catalase activity were determined in plasma using a spectrophotometric approach following the instructions given in the kits, which were purchased from BioAssay Systems, USA (Cat # DTAC-100 and # ECAT-100). Blood testosterone levels were analyzed by ELISA following the instructions provided with the kit (DRG, Cat # EIA-5179). Rat cytokine/chemokine profiles, including those of the rat adipocytokine leptin, interleukin-6 (IL-6), interleukin-1 beta (IL-1β), monocyte chemoattractant protein-1 (MCP-1, CCL2) and tumor necrosis factor-alpha (TNF-α), were analyzed in plasma samples with a Milliplex rat-cytokine immunoassay kit (Millipore, USA). Cat # RECYTMAG-65 K-08) according to the manufacturer's instructions.

Anuradha R., Srinivas M., Satyavani M., Suresh K., Muralidhar M.N, & Rajender Rao K. (2024). Preconceptional paternal caloric restriction of high-fat diet-induced obesity in Wistar rats dysregulates the metabolism of their offspring via AMPK/SIRT1 pathway. Lipids in health and disease, 23(1).

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