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Glutamate determination kit

Manufactured by Merck Group
Sourced in United States

The Glutamate Determination Kit is a laboratory product designed to quantify the concentration of glutamate in a sample. It provides a simple and reliable method for the colorimetric determination of glutamate levels.

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2 protocols using glutamate determination kit

1

Zika Virus E-Protein Induces Glutamate Release

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Astrocytes were transfected with ZIKV E-protein and respective empty vector (control) for 24 h, followed by collection of supernatants. It was further processed for the detection of glutamate release using glutamate determination kit (Sigma Aldrich, USA, Cat# GLN1) as per manufacturer’s protocol.
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2

Glutamine and Glutamate Quantification

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Glutamine and a Glutamate Determination Kit (Sigma‐Aldrich) were used to determine the concentration of glutamine and glutamate in cells. Cells (1 × 104/well) were divided into two parts: one part was measured with glutaminase, which was used to convert glutamine to glutamate, and the other part was measured directly. A spectrophotometer (Bio‐Rad) was used to measure the results at 340 nm.
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