All reagents including sphingosine-1-phosphate (S1P) were purchased from Sigma-Aldrich unless otherwise specified. Imatinib was obtained commercially from LC Laboratories and reconstituted in DI water to yield a 20 mM stock. Dulbecco’s phosphate buffered saline (PBS) and trypsin were purchased from Life Technologies, while biological grade glutaraldehyde and paraformaldehyde used for cell fixation were obtained from Electron Microscopy Science. The following reagents and antibodies were acquired for immunofluorescence: mouse monoclonal anti-cortactin (p80/85) antibody clone 4F11 (EMD Millipore), mouse anti-paxillin antibody clone 349 (BD Biosciences), mouse anti-VE-cadherin antibody (Santa Cruz), Alex Fluor 488 goat anti-mouse IgG secondary antibody (Life Technologies), and Rhodamine-conjugated phalloidin (Molecular Probes).
Alex fluor 488 goat anti mouse igg secondary antibody
Manufactured by Thermo Fisher Scientific
The Alexa Fluor 488 goat anti-mouse IgG secondary antibody is a fluorescently labeled antibody used to detect mouse primary antibodies in various immunoassays and imaging applications. The Alexa Fluor 488 dye provides bright green fluorescence upon excitation at 488 nm.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin, by Thermo Fisher Scientific (2 mentions)
Dulbecco s phosphate buffered saline, by Thermo Fisher Scientific (2 mentions)
Biological grade glutaraldehyde, by Electron Microscopy Sciences (2 mentions)
Rhodamine conjugated phalloidin, by Thermo Fisher Scientific (2 mentions)
Mouse monoclonal anti cortactin p80 85 antibody clone 4f11, by Merck Group (2 mentions)
Mouse anti paxillin antibody clone 349, by BD (2 mentions)
Mouse anti ve cadherin antibody, by Santa Cruz Biotechnology (2 mentions)
Paraformaldehyde (pfa), by Electron Microscopy Sciences (2 mentions)
Sphingosine 1 phosphate (s1p), by Merck Group (1 mentions)
Imatinib, by LC Laboratories (1 mentions)
Hrp conjugated β actin antibody, by Proteintech (1 mentions)
Xfect rna transfection reagent, by Takara Bio (1 mentions)
2 protocols using alex fluor 488 goat anti mouse igg secondary antibody
1
Cytoskeletal Dynamics in Endothelial Cells
2
Endothelial Cell Cytoskeleton Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Reagents were purchased from Sigma-Aldrich (e.g., S1P, thrombin) except as noted below. Dulbecco’s phosphate buffered saline (PBS) and trypsin were obtained from Life Technologies, while biological grade glutaraldehyde and paraformaldehyde were obtained from Electron Microscopy Science. The following reagents and antibodies were acquired for immunofluorescence: mouse monoclonal anti-cortactin (p80/85) antibody clone 4F11 (EMD Millipore), mouse anti-paxillin antibody clone 349 (BD Biosciences), mouse anti-VE-cadherin antibody (Santa Cruz), Alex Fluor 488 goat anti-mouse IgG secondary antibody (Life Technologies), HRP-conjugated β-actin antibody (Proteintech Group Inc), and Rhodamine-conjugated phalloidin (Molecular Probes). Scrambled and c-Abl specific siRNA was obtained from Santa Cruz Biotechnology, and Xfect RNA transfection reagent was obtained from Takara Clontech.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!