Cd45.2 bv785

Manufactured by BioLegend

CD45.2-BV785 is a fluorochrome-conjugated antibody that recognizes the CD45.2 antigen, a cell surface marker expressed on various hematopoietic cells. It is designed for use in flow cytometry applications.

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2 protocols using cd45.2 bv785

OT-II T-Cell Trafficking in Ptpn22 Mice

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1 x 10⁶ CTV labelled CD4⁺ TCR Vα2⁺ Vβ5⁺ WT OT-II T-cells were injected intravenously into WT or Ptpn22^-/- mice. The following day mice were immunised with ovalbumin (10 μg/mouse) or PBS via intraperitoneal injection. After 72 hours spleens were harvested and single cell suspensions were stained using antibodies against: TCR Vα2-PE (clone MR9-4; Biolegend), TCR Vβ5-APC (clone B20.1; Biolegend), CD4-APC/Cy7 (clone GK1.5; Biolegend), CD45.1-PE/Cy7 (clone A20; Biolegend), CD45.2-BV785 (clone 104; Biolegend). CTV dilution was determined within the live, singlet, CD4⁺, Vα5⁺Vβ2⁺, CD45.1⁺, CD45.2^- population.

Clarke F., Jordan C.K., Gutiérrez-Martinez E., Bibby J.A., Sanchez-Blanco C., Cornish G.H., Dai X., Rawlings D.J., Zamoyska R., Guermonprez P., Cope A.P, & Purvis H.A. (2017). Protein tyrosine phosphatase PTPN22 is dispensable for dendritic cell antigen processing and promotion of T-cell activation by dendritic cells. PLoS ONE, 12(10), e0186625.

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Multicolor Flow Cytometry of Immune Cells

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Flow cytometry data were acquired using a Cytek Aurora equipped with violet (405 nm), blue (488 nm), and red (640 nm) lasers. The following antibodies and stains were used: CCR2-BV421 (clone 475301, 747963; BD), Ly6C-BV510 (clone HK1.4, 128033; Biolegend), B220-BV605 (clone RA3-6B2, 103243; Biolegend), Ly6G-BV711 (clone 1A8, 127643; Biolegend), CD11c-BV785 (clone N418, 117336; Biolegend), CX3CR1-A488 (clone SA011F11, 149021; Biolegend), CX3CR1-BV785 (clone SA011F11, 149029; Biolegend), CD163-PE (clone TNKUPJ, 12-1631-80; Thermo Fisher Scientific), CD64-PE/Cy7 (clone X54-5/7.1, 139313; Biolegend), MRC1-PCP5.5 (clone C068C2, 141716; Biolegend), CD11b-PEFire640 (clone M1/70, 101279; Biolegend) TCRb-APC (clone H57-597, 109212; Biolegend), MHCII-A700 (clone M5/114.15.2, 107622; Biolegend), XCR1-APC/Cy7 (clone ZET, 148223; Biolegend), CD45-APC-Fire810 (clone 30F11, 103173; Biolegend), CD45.1-A647 (clone A20, 110720; Biolegend), CD45.2- BV785 (clone 104, 109839; Biolegend), and LIVE/DEAD Violet dead cell stain kit (L34955; Thermo Fisher Scientific). Flow cytometry data was analyzed using Flowjo 10 software. Dimensionality reduction and cluster identification were performed using the UMAP and Phenograph packages, respectively.

Lund H., Hunt M.A., Kurtović Z., Sandor K., Kägy P.B., Fereydouni N., Julien A., Göritz C., Vazquez-Liebanas E., Andaloussi Mäe M., Jurczak A., Han J., Zhu K., Harris R.A., Lampa J., Graversen J.H., Etzerodt A., Haglund L., Yaksh T.L, & Svensson C.I. (2023). CD163+ macrophages monitor enhanced permeability at the blood–dorsal root ganglion barrier. The Journal of Experimental Medicine, 221(2), e20230675.

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