Anti esrp1

We performed western blotting as described previously [39 (link)]. The following primary antibodies were used for protein detection: anti-TET2 (catalog number 61390, Active Motif, Carlsbad, CA), anti-ESRP1 (catalog number GTX131373, GeneTex, Irvine, CA, USA), anti-CD44 (catalog number MAB7045, R&D Systems, Minneapolis, MN, USA), anti-ADARB2 (catalog number sc-73410, Santa Cruz Biotechnology, Dallas, TX, USA), anti-ZEB1 (catalog number 3396, Cell Signaling Technology, Danvers, MA, USA), anti-GRHL2 (catalog number HPA004820, Sigma-Aldrich, St. Louis, MO), and anti-vimentin (catalog number 3932, Cell Signaling Technology). The ECL prime blocking agent (GE Healthcare Life Sciences, Piscataway, NJ, USA) was used for blocking and Lumigen TMA-6 reagents for detection (Lumigen, Inc., Southfield, MI, USA). The blots were re-probed with an HSP90 antibody (catalog number 4875, Cell Signaling Technology) or with an anti-β-actin antibody (catalog number A5316, Sigma-Aldrich) to document equal protein loading. Each western blot was performed at least twice; representative blots are shown. Relative intensities of the bands were determined with ImageJ software (National Institutes of Health, Bethesda, MD, USA).

We performed Western blotting, involving detection of protein bands as enhanced chemo-luminescence signal on X-ray films, as described previously [25 (link)]. The following primary antibodies were used for protein detection: anti-METTL3 (catalog number PA5-28178 ThermoFisher Scientific, Waltham, MA, USA), anti-ESRP1 (catalog number GTX131373, GeneTex, Irvine, CA, USA), (catalog number ab155227, Abcam, Cambridge, MA, USA), anti-CD44 (catalog number MAB7045, R&D Systems, Minneapolis, MN, USA), anti-SNAIL1 (catalog number 3895, Cell Signaling Technology, Danvers, MA, USA), and anti-vimentin (catalog number 3932, Cell Signaling Technology). Each Western blot was performed at least twice; representative blots are shown. We quantified relative intensities of protein bands detected on X-ray films with ImageJ software (National Institutes of Health, Bethesda, MD, USA).