Human pol β was expressed and purified44 (link). Chlodronate, etidronate, imidodiphosphate, pamidronate, and pyrophosphate were from Sigma-Aldrich. The β,γ-imido modified nucleoside triphosphate analog, 2′-deoxyguanosine-5′-[(β,γ)-imido]triphosphate (dGMPPNP), was from Jena Bioscience. Chain terminating nucleoside triphosphates; ddCTP was from GE Healthcare, 3′-azido-2′,3′-dideoxythymidine triphosphate (AZTTP) and arabinofuranosylcytosine triphosphate (araCTP) were from Trilink Biotechnologies, and gemcitabine (dFdCTP) was obtained from Jena Bioscience. [α-35S]dATP, [α-32P]dCTP, and [32P]PPi were from Perkin Elmer. Polyethyleneimine (PEI) cellulose thin layer chromatography (TLC) plates containing a fluorescent indicator were purchased from EMD Millipore.
Polyethyleneimine pei cellulose thin layer chromatography plates
Manufactured by Merck Group
Polyethyleneimine (PEI) cellulose thin layer chromatography (TLC) plates are a type of laboratory equipment used for separating and analyzing chemical compounds. These plates consist of a thin layer of PEI-cellulose material coated onto a solid support, typically glass or aluminum. The PEI-cellulose material serves as the stationary phase, and the compounds being analyzed are separated based on their relative affinities for the stationary phase and the mobile phase (typically a solvent) that is used.
Automatically generated - may contain errors
Lab products found in correlation
Etidronate, by Merck Group (2 mentions)
Pamidronate, by Merck Group (2 mentions)
Imidodiphosphate, by Merck Group (2 mentions)
32p ppi, by PerkinElmer (2 mentions)
Ddctp, by GE Healthcare (2 mentions)
Chlodronate, by Merck Group (2 mentions)
3 azido 2 3 dideoxythymidine triphosphate azttp, by TriLink (2 mentions)
Arabinofuranosylcytosine triphosphate aractp, by TriLink (2 mentions)
Gemcitabine dfdctp, by Jena Biosciences (2 mentions)
α 35s datp, by PerkinElmer (2 mentions)
α 32p dctp, by PerkinElmer (2 mentions)
2 protocols using polyethyleneimine pei cellulose thin layer chromatography plates
1
Human pol β purification and assay
2
Human pol β purification and assay
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Human pol β was expressed and purified44 (link). Chlodronate, etidronate, imidodiphosphate, pamidronate, and pyrophosphate were from Sigma-Aldrich. The β,γ-imido modified nucleoside triphosphate analog, 2′-deoxyguanosine-5′-[(β,γ)-imido]triphosphate (dGMPPNP), was from Jena Bioscience. Chain terminating nucleoside triphosphates; ddCTP was from GE Healthcare, 3′-azido-2′,3′-dideoxythymidine triphosphate (AZTTP) and arabinofuranosylcytosine triphosphate (araCTP) were from Trilink Biotechnologies, and gemcitabine (dFdCTP) was obtained from Jena Bioscience. [α-35S]dATP, [α-32P]dCTP, and [32P]PPi were from Perkin Elmer. Polyethyleneimine (PEI) cellulose thin layer chromatography (TLC) plates containing a fluorescent indicator were purchased from EMD Millipore.
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