A homebuilt two-photon excitation fluorescence microscope was used for microendoscopic imaging and was described in detail previously (Ji et al., 2010 (link)). 940 nm output from a femtosecond pulsed laser source with a built-in dispersion compensation unit (InSight DeepSee, Spectra Physics, CA, USA) was used to provide two-photon excitation. A 10×/0.45NA microscope objective (CFI Plan Apo Lambda 10X, Nikon, Tokyo, Japan) was used in conjunction with 0.5-NA singlet GRIN lenses, whereas a 4×/0.2NA objective (CFI Plan Apo Lambda 4X, Nikon) was used with doublet GRIN lenses to match their NA on the image side. Microscope control and data acquisition were executed using custom-written LabVIEW software.
Cfi plan apo lambda 10x
Manufactured by Nikon
Sourced in Japan
The CFI Plan Apo Lambda 10X is an objective lens designed for microscopy applications. It features a plan-apochromatic optical design and a magnification of 10X. The lens is suitable for a wide range of microscopy techniques.
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Lab products found in correlation
2 protocols using cfi plan apo lambda 10x
1
Two-Photon Excitation Fluorescence Microscope for Microendoscopic Imaging
2
Multimodal Imaging of Cell Structures
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The following microscopes were used: (1) Zeiss LSM 880 inverted laser scanning confocal microscope equipped with a plan-apochromatic 63x oil objective lens (Zeiss, NA = 1.4), 40x oil objective lens (Zeiss, NA = 1.3), 405 nm, 488 nm and 561 nm laser lines, and ZEN software (Zeiss, Oberkochen, Germany). (2) Nikon Eclipse TiE inverted microscope equipped with a CFI Plan Apo Lambda 10x (Nikon Instruments, NA = 0.45) and a CFI Plan Apo VC 60x oil objective lens (Nikon Instruments, NA = 1.4), sCMOS camera (Zyla 4.2, Andor), SPECTRA X light engine illumination source (Lumencor, Beaverton, OR), and NIS-Elements software (Nikon Instruments, Tokyo, Japan). The experimental details for each imaging experiment are provided in the appropriate subsection in Materials and Methods.
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