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Sybr premix ex taii

Manufactured by Takara Bio
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Sourced in Japan

SYBR Premix Ex TaII is a pre-mixed solution for real-time PCR (qPCR) and reverse transcription-PCR (RT-qPCR) applications. It contains all the necessary components, including SYBR Green I, a fluorescent dye that binds to double-stranded DNA, and the ExTaq DNA polymerase, which enables efficient amplification of target sequences.

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Lab products found in correlation

Oligo dt primer, by Takara Bio (1 mentions) Superscript 3 reverse transcriptase, by Takara Bio (1 mentions) Trizol reagent, by Takara Bio (1 mentions) 7500 real time pcr system, by Thermo Fisher Scientific (1 mentions) Rnaiso plus, by Takara Bio (1 mentions) Primescript rt reagent kit with gdna eraser, by Takara Bio (1 mentions)

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SYBR Premix (174 citations)

2 protocols using sybr premix ex taii

1

Quantifying Gene Expression in Adipocytes

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Total RNA was isolated from 3T3-L1 adipocytes and epididymal adipose tissue from mice using Trizol reagent (TAKARA, Ohtsu, Japan). Reverse transcription was performed using SuperScript III Reverse transcriptase and Oligo (dT) primer (TAKARA, Ohtsu, Japan). PCR amplification was performed with SYBR Premix Ex TaII (TAKARA, Ohtsu, Japan) using the Applied Biosystems 7500 Real-Time PCR System. Fold changes in expression were analyzed using the 2−ΔΔ Ct relative quantitative method. This Primers used in the study were included in Supplementary Tabel S1.
Shan Y., Zhang S., Gao B., Liang S., Zhang H., Yu X., Zhao J., Ye L., Yang Q, & Shang W. (2020). Adipose Tissue SIRT1 Regulates Insulin Sensitizing and Anti-Inflammatory Effects of Berberine. Frontiers in Pharmacology, 11, 591227.
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2

Cardiac RNA Extraction and qPCR

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The RNAiso Plus (Takara, Japan) was utilized to extract total RNA from heart tissues for qPCR. Reverse transcription was performed using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Japan). The SYBR Premix Ex Ta II (Takara, Japan) was employed for qPCR following the manufacturer's guidelines. The primer sequences can be found in Table S1. The cycling temperature for 40 cycles was adjusted to 95°C for 15 seconds, followed by 55°C for 15 seconds, and then 72°C for 15 seconds. β-Actin was utilized as an internal reference for control purposes.
Feng R., Wang D., Li T., Liu X., Peng T., Liu M., Ren G., Xu H., Luo H., Lu D., Qi B., Zhang M, & Li Y. (2024). Elevated SLC40A1 impairs cardiac function and exacerbates mitochondrial dysfunction, oxidative stress, and apoptosis in ischemic myocardia. International Journal of Biological Sciences, 20(2), 414-432.
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