Ldh kit

Manufactured by Thermo Fisher Scientific

Sourced in United States

The LDH kit is a laboratory assay used to measure the activity of the enzyme lactate dehydrogenase (LDH) in biological samples. LDH is an enzyme found in various tissues and is released into the bloodstream when cells are damaged, making it a useful marker for certain medical conditions.

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Pierce Lactate Dehydrogenase (LDH) Cytotoxicity Assay Kit (20 citations) Pierce LDH assay kit (13 citations) Lactate dehydrogenase (LDH) Cytotoxicity Assay Kit (11 citations) LDH cytotoxicity kit (10 citations) LDH cytotoxicity detection kit (6 citations) Pierce LDH Cytotoxicity Detection Kit (4 citations) Invitrogen CyQuant LDH Cytotoxicity Assay Kit (3 citations) LDH toxicity assay kit (3 citations) Pierce lactic dehydrogenase (LDH) cytotoxicity assay kit (2 citations) LDH release assay kit (2 citations)

3 protocols using ldh kit

LDH Release Assay for Cytotoxicity Evaluation

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The rate of LDH
release was determined
with a commercial LDH kit supplied by Thermo Fisher Scientific Inc.
(Pittsburgh PA, US). Briefly, H9c2 cells were inoculated in a 96-well
plate and grew to about 70% confluence. After the indicated treatments,
50 μL of supernatant per well was carefully removed and transferred
into corresponding wells for the determination of extracellular LDH
levels. Then, 100 μL of DMEM with 2% Triton X-100 was added
to the adherent cells to lyse the cells. Fifty microliters of cell
lysate was transferred to a 96-well plate to determine intracellular
LDH levels. The same volume of prepared reaction mixture was added
to the supernatant or homogenate, separately, and reacted for 10 min
at room temperature by gentle shaking. Stopping solution was added
to stop the reaction. Absorbance was measured at 490 and 600 nm with
a microplate reader (Tecan Infinite F200, Switzerland). LDH release
rate was calculated as follows (experiments were performed 4 times):

Zhao Y., Yang C., Organ C., Li Z., Bhushan S., Otsuka H., Pacheco A., Kang J., Aguilar H.C., Lefer D.J, & Xian M. (2015). Design, Synthesis, and Cardioprotective Effects of N-Mercapto-Based Hydrogen Sulfide Donors. Journal of Medicinal Chemistry, 58(18), 7501-7511.

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Standard Chemical Reagents and Cell Culture

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All
chemicals and solvents were purchased
from Sigma-Aldrich, VWR, or Iris Biotech and were used as received.
Acetonitrile was distilled under inert atmosphere before use. MilliQ
water (Millipore, resistivity = 18.2 mΩ cm) was used throughout.
DMEM supplemented with 10% FCS, penicillin (50 UI/mL), and streptomycin
(0.05 mg/mL) was purchased by Life Technologies. The LDH kit was purchased
from Thermo Fisher Scientific.

Zanna N., Focaroli S., Merlettini A., Gentilucci L., Teti G., Falconi M, & Tomasini C. (2017). Thixotropic Peptide-Based Physical Hydrogels Applied to Three-Dimensional Cell Culture. ACS Omega, 2(5), 2374-2381.

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Chow Diet Impacts Cholesterol Homeostasis

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Chow diet (Harlan Teklad LM-485) was purchased from ICMR-NIN, Hyderabad, India. Dulbecco′s modified Eagle′s medium (DMEM) and foetal calf serum (FCS) were purchased from GIBCO (Waltham, MA, USA). Gentamycin, BCA protein assay kit, apoptosis kit, LDH kit, CFSE kit was purchased from Thermofisher (Waltham, MA, USA). Triton X100, sodium butyrate, sodium propionate, sodium acetate, Hoechst 33342, Laurdan, CTX-B-FITC, water soluble cholesterol (MBCD-cholesterol) was purchased from Sigma (St. Louis, MO, USA). Amplex red cholesterol assay kit, Trizol, Fillipin was purchased from Invitrogen (Carlsbad, CA, USA). Cholesterol, Cholesterol analogue (4-cholesten-3-one) and Phosphatidylcholine were obtained from Avanti polar lipids. Anti CD44-FITC antibody was purchased from BD Pharmigen^™. Anti CD71-biotin was purchased from Bioss (Massachusetts, Boston, USA). Secondary Steptavidin PE was purchased from Biolegend (San Diego, CA, USA). Super Reverse Transcriptase MuLV Kit, RT [2 (link)] SYBR^® Green qPCR Mastermix, were purchased from Qiagen (Hilden, Germany). Protease inhibitor was purchased from Takara. Primers were purchased from IDT (Germany). RAW264.7 cell line was purchased from ATCC (USA).

Das O., Masid A., Chakraborty M., Gope A., Dutta S, & Bhaumik M. (2023). Butyrate driven raft disruption trots off enteric pathogen invasion: possible mechanism of colonization resistance. Gut Pathogens, 15, 19.

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