Pmax plasmid

Manufactured by Lonza

Sourced in Switzerland

The PMAX plasmid is a versatile genetic vector used in molecular biology and biotechnology applications. It serves as a DNA-based platform for the expression and manipulation of genes in various cell types. The core function of the PMAX plasmid is to enable the efficient and stable introduction of genetic material into target cells, facilitating the study and utilization of specific genes and their products.

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Lab products found in correlation

Human t cell nucleofector kit, by Lonza (1 mentions) Codon optimization tool, by Integrated DNA Technologies (1 mentions)

Spelling variants of this product

Pmax-GFP plasmids (2 citations) GFP pMAX plasmid (2 citations)

2 protocols using pmax plasmid

Transfection of AhR siRNA and BATF cDNA

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Predesigned siRNA for ahr and its negative control siRNA were obtained from Life technologies (Grand Island, NY). Human BATF cDNA (Genescript, Piscatway, NJ) was inserted to pMAX plasmid (Lonza, Allendale, NJ). Transfection was performed by electroporation using human T cell Nucleofector Kit (Lonza, Allendale, NJ).

Takami M., Fujimaki K., Nishimura M, & Iwashima M. (2015). AhR is a molecular target of Calcitriol in human T cells. Journal of immunology (Baltimore, Md. : 1950), 195(6), 2520-2523.

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Cloning and Characterization of GFP-ENPL Fusion Protein

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Amino acid sequences of rat (Q66HD0) and mouse (P08113) ENPL were obtained from the UniProt database (https://www.uniprot.org/). The sequences were fit by Clustal Omega (https://www.ebi.ac.uk/Tools/msa/clustalo/) and Protein Blast (https://blast.ncbi.nlm.nih.gov/Blast.cgi) accessed on 3 January 2020. Altogether, a similarity of 98.01% was found between the ENPL primary amino acid sequences in the two species (Supplementary Figure S1). The Q66HD0 rat ENPL sequence was used in our work. Based on the protein sequence, codon optimization was performed with Codon Optimization Tool of Integrated DNA Technologies, Inc. (https://eu.idtdna.com/) accessed on 3 January 2020. A GGSGGGSG linker was inserted between the GFP and ENPL sequences. The gene encoding the GFP-ENPL fusion protein was synthetized by Bio Basic Inc., (Markham, ON, Canada) and was inserted into a pMAX plasmid (Lonza, Basel, Switzerland) for in vitro expression. The DNA sequence of the pMAXGFP-ENPL is available in Supplementary Figure S2.

Koncz A., Turiák L., Németh K., Lenzinger D., Bárkai T., Lőrincz P., Zelenyánszki H., Vukman K.V., Buzás E.I, & Visnovitz T. (2023). Endoplasmin Is a Hypoxia-Inducible Endoplasmic Reticulum-Derived Cargo of Extracellular Vesicles Released by Cardiac Cell Lines. Membranes, 13(4), 431.

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