Mouse monoclonal α his

Electroporation and preparation of sub cellular fractions from M. smegmatis and M.tb H37Ra was done as described for GAPDH. Samples (40 µg each) were analyzed on a 10 % SDS-PAGE, control fractions of untransformed M. smegmatis and M.tb H37Ra were run alongside. Resolved proteins were either stained with coomassie blue or were transferred onto nitrocellulose membrane. Blots were probed with mouse monoclonal α-His (Sigma) for 1 h followed by incubation with goat α-mouse IgG-HRP (1:10,000 for 1 h), washed and developed with TMB/H₂O₂.
Proteins have been referred to as EC-PykA-His, MS-PykA-H, MT-PykA-H and EC-H-Enolase, MS-H-Enolase, MT-H-Enolase to reflect the expression host, position of tag and protein.

mouse monoclonal α-CYCS (BD Pharmingen, Franklin Lakes, USA, Cat # 556432), goat polyclonal α-ENO (Santa Cruz Biotechnology, Inc, Santa Cruz, USA, Cat # sc-7455), mouse monoclonal α-His (Sigma-Aldrich, Saint Louis, USA, Cat # SAB1305538), mouse monoclonal α-LRPPRC (Santa Cruz Biotechnology, Inc, Santa Cruz, USA, Cat # sc-166178), rabbit polyclonal α-OPA1 (Proteintech Group, Rosemont, USA, Cat # 27733-1-AP), rabbit polyclonal α-PNPase (Abcam, Cambridge, UK, Cat # ab96176), mouse monoclonal α-Ro60 (Santa Cruz Biotechnology, Inc, Santa Cruz, USA, Cat # sc-100844), rabbit polyclonal α-Ro60 (Sigma-Aldrich, Saint Louis, USA, Cat # HPA002835), rabbit polyclonal α-TOMM20 (Santa Cruz Biotechnology, Inc, Santa Cruz, USA, Cat # sc-11415), rabbit polyclonal α-uL4m (Proteintech Group, Rosemont, USA, Cat # 27484-1-AP), polyclonal sheep HRP-conjugated α-mouse (GE Healthcare, Chicago, USA, Cat # NXA931), polyclonal donkey HRP-conjugated α-rabbit (GE Healthcare, Chicago, USA, Cat # NA934V).