Immunofluorescence and immunohistochemistry assays were used to detect the expression of PTEN and the status of apoptosis of collected tissue samples, respectively. In brief, for immunohistochemistry assays, the tissue samples were fixed by using 4% of formaldehyde (Sigma Aldrich), deparaffinized and rehydrated using gradient alcohol. In the next step, the sections were stained with anti‐PTEN primary antibody (Abcam) and suitable biotin‐labelled secondary antibody (Thermo Fisher Scientific) before the relative PTEN expression in each sample was evaluated under a Zeiss LSM700 confocal laser‐scanning microscope in accordance with the instruction provided by the manufacturer. For immunofluorescence assays, the status of apoptosis of collected tissue samples was evaluated by using a Vybrant Apoptosis Assay Kit (Thermo Fisher Scientific) in accordance with the routine assay protocol provided by the kit manufacturer.
Anti pten primary antibody
Manufactured by Abcam
The Anti-PTEN primary antibody is a reagent used for the detection and analysis of the PTEN protein in various experimental applications. PTEN is a tumor suppressor protein that plays a crucial role in regulating cellular processes. This antibody can be used to identify and quantify PTEN expression levels in biological samples.
Automatically generated - may contain errors
2 protocols using anti pten primary antibody
1
Evaluating PTEN Expression and Apoptosis
2
Evaluation of PTEN and Apoptosis in Tissues
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Immuno uorescence and immunohistochemistry assays were used to detect the expression of PTEN and the status of apoptosis of collected tissue samples, respectively. In brief, for immunohistochemistry assays, the tissue samples were xed by using 4% of formaldehyde (Sigma Aldrich, St. Louis, MO), depara nized, and rehydrated using gradient alcohol. In the next step, the sections were stained with anti-PTEN primary antibody (Abcam, Cambridge, MA) and suitable biotin-labeled secondary antibody (Thermo Fisher Scienti c, Waltham, MA) before the relative PTEN expression in each sample was evaluated under a Zeiss LSM700 confocal laser-scanning microscope in accordance with the instruction provided by the manufacturer. For immuno uorescence assays, the status of apoptosis of collected tissue samples was evaluated by using a Vybran Apoptosis Assay Kit (Thermo Fisher Scienti c, Waltham, MA) in accordance with the routine assay protocol provided by the kit manufacturer.
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