Tacedinaline

Manufactured by Selleck Chemicals

Sourced in Canada

Tacedinaline is a chemical compound used as a laboratory reagent. It functions as a histone deacetylase (HDAC) inhibitor, which can be utilized in various research applications.

Automatically generated - may contain errors

Lab products found in correlation

Rgfp966, by Selleck Chemicals (2 mentions) Rgfp109, by Selleck Chemicals (1 mentions) Tubastatin a, by Cayman Chemical (1 mentions) Mouse anti flag m2, by Merck Group (1 mentions) Rabbit anti α tubulin, by Abcam (1 mentions) Cy5 conjugated donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Alexa fluor 488 conjugated affinipure donkey anti mouse igg, by Jackson ImmunoResearch (1 mentions) Hrp conjugated goat anti mouse, by Jackson ImmunoResearch (1 mentions) Donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Cy3 conjugated donkey anti mouse igg, by Jackson ImmunoResearch (1 mentions) Tasquinimod, by Selleck Chemicals (1 mentions) Ultrapure lipopolysaccharide lps, by List Biological Laboratories (1 mentions) Pci 34051, by Selleck Chemicals (1 mentions) Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions) Decitabine, by Selleck Chemicals (1 mentions) Qgp 1 cell line, by Japanese Collection of Research Bioresources Cell Bank (1 mentions) Nci h727 cell line, by American Type Culture Collection (1 mentions)

3 protocols using tacedinaline

Immunofluorescence and Western Blotting Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary antibodies were: mouse anti-human HSP70, specific for stress-inducible HSPA1A (StressMarq, Canada, SMC-100B; 1:100 ICC; 1:1000 WB), mouse anti-Flag M2 (Sigma-Aldrich, #F1804; 1:400 ICC); rabbit anti-FUS (Proteintech, USA, 11570-1-AP; 1:400 ICC), mouse anti-human SOD1 (Sigma-Aldrich Canada, SD-G6; 1:100 ICC), rabbit anti-acetyl-histone H3K9/K14 (Cell Signaling, #9677; 1:400 ICC; 1:1000 WB), mouse anti-GAPDH (MediMabs, Canada, #MM-0163; 1:1000 WB), moue monoclonal anti-acetylated tubulin (Sigma-Aldrich #T6793; 1:1000 WB) and rabbit anti-α-tubulin (Abcam #ab15246; 1:1000 WB).
Secondary antibodies (Jackson Immunoresearch: Cedarlane, Canada): Alexa Fluor 488-conjugated Affinipure donkey anti-mouse IgG (1:300); Cy3-conjugated donkey anti-mouse IgG 1:300); Cy5-conjugated donkey anti-rabbit IgG (1:300), and HRP-conjugated goat anti-mouse (1/5000 for WB, 1/500 for IC) or donkey anti-rabbit IgG (Jackson Immunoresearch (1:2500).
HDAC inhibitors and HSP-inducing drugs: SAHA (suberoylanilide hydroxamic acid) and tubastatin A (Cayman Chemical: Cedarlane, Canada); tacedinaline, RGFP109 and RGFP966 (Selleckchem: Cedarlane, Canada); sodium phenylbutyrate (Selleckchem); arimoclomol (Toronto Research Chemicals, Canada); NXD30001 was previously supplied by NexGenix Pharmaceuticals (Cha et al. 2014 (link)).

Kuta R., Larochelle N., Fernandez M., Pal A., Minotti S., Tibshirani M., St. Louis K., Gentil B.J., Nalbantoglu J.N., Hermann A, & Durham H.D. (2020). Depending on the stress, histone deacetylase inhibitors act as heat shock protein co-inducers in motor neurons and potentiate arimoclomol, exerting neuroprotection through multiple mechanisms in ALS models. Cell Stress & Chaperones, 25(1), 173-191.

+ Open protocol

+ Expand

Cell Culture and Reagent Acquisition

Check if the same lab product or an alternative is used in the 5 most similar protocols

Roswell Park Memorial Institute (RPMI) 1640 and Dulbecco’s Modified Eagle Medium (DMEM) was purchased from Invitrogen (Carlsbad, CA.). Ultrapure Lipopolysaccharide (LPS) was purified from Salmonella minnesota R595 (List Biological Laboratories). TBWCL was obtained from BEI resources. RGFP966, tacedinaline, tasquinimod, HPOB and PCI34051 were purchased from Selleckchem.

Campo M., Heater S., Peterson G.J., Simmons J.D., Skerrett S.J., Mayanja-Kizza H., Stein C.M., Boom W.H, & Hawn T.R. (2021). HDAC3 inhibitor RGFP966 controls bacterial growth and modulates macrophage signaling during Mycobacterium tuberculosis infection. Tuberculosis (Edinburgh, Scotland), 127, 102062.

+ Open protocol

+ Expand

Characterization of Neuroendocrine Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human pancreatic NET BON1 cell line (14) was kindly provided by Prof. Rüdiger Göke, University of Marburg. The pancreatic islet tumor QGP1 cell line (14) was originally obtained from the Japanese Collection of Research Bioresources Cell Bank. The human, bronchopulmonary neuroendocrine NCI-H727 cell line (14) was obtained from the American Type Culture Collection. The human midgut carcinoid cell line GOT1 (14, 15) was kindly provided by Prof. Ola Nilsson, Sahlgrenska University Hospital. All cell lines were certified for identity by short-tandem repeat (STR) typing, performed by the German Biologic Resource Centre DSMZ. All cell lines used in this study were cultured and incubated as previously described (16) .
5-FU, decitabine, and tacedinaline were purchased from Selleckchem, dissolved in dimethyl sulfoxide, and stored at 220°C. 68 Ga-DOTATOC was prepared using SomaKit TOC purchased from Advanced Accelerator Applications SA. 68 Ga was obtained by elution of a GalliaPharm 68 Ge/ 68 Ga generator by Eckert and Ziegler.

Jin X.F., Auernhammer C.J., Ilhan H., Lindner S., Nölting S., Maurer J., Spöttl G, & Orth M. (2019). Combination of 5-Fluorouracil with Epigenetic Modifiers Induces Radiosensitization, Somatostatin Receptor 2 Expression, and Radioligand Binding in Neuroendocrine Tumor Cells In Vitro. Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 60(9).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!