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Egfr1

Manufactured by Abcam

EGFR1 is a recombinant protein that represents the extracellular domain of the human epidermal growth factor receptor (EGFR) protein. EGFR is a cell surface receptor tyrosine kinase that plays a key role in cell signaling pathways involved in cell growth and proliferation.

Automatically generated - may contain errors

2 protocols using egfr1

1

Immunohistochemical Analysis of CRC Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumoural and non-neoplastic tissue sections from CRC patients were subjected to immunohistochemical staining (Fig. 3a and Supplementary Fig. 24). Primary monoclonal antibodies against EGFR (EGFR1, Abcam, 1:50 dilution), EpCAM (VU-1D9, Abcam, 1:100 dilution), and CD24 (eBioSN3, ebioscience, 1:100 dilution) were used and the staining was conducted on a Ventana BenchMark XT automated slide stainer (Ventana Medical Systems, Inc.), according to the manufacturer’s recommendations. Stained images were scored, based on the fraction of positive cells among total cancer cells, by a pathologist (G.Y.) who was blind to clinicopathological variables and EV profiling results. Positive cells were defined by positively stained cytoplasmic or membranous pattern within cancer cells in the face of concurrent negative labelling in non-neoplastic tissues. The marker expression was ranked from 0 (negative) to 1 (positive) with the increment of 0.1; this level was used as an ordinal variable in Spearman’s rank test with EV profiling results.
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2

Immunohistochemical Analysis of CRC Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumoural and non-neoplastic tissue sections from CRC patients were subjected to immunohistochemical staining (Fig. 3a and Supplementary Fig. 24). Primary monoclonal antibodies against EGFR (EGFR1, Abcam, 1:50 dilution), EpCAM (VU-1D9, Abcam, 1:100 dilution), and CD24 (eBioSN3, ebioscience, 1:100 dilution) were used and the staining was conducted on a Ventana BenchMark XT automated slide stainer (Ventana Medical Systems, Inc.), according to the manufacturer’s recommendations. Stained images were scored, based on the fraction of positive cells among total cancer cells, by a pathologist (G.Y.) who was blind to clinicopathological variables and EV profiling results. Positive cells were defined by positively stained cytoplasmic or membranous pattern within cancer cells in the face of concurrent negative labelling in non-neoplastic tissues. The marker expression was ranked from 0 (negative) to 1 (positive) with the increment of 0.1; this level was used as an ordinal variable in Spearman’s rank test with EV profiling results.
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