Immunoprecipitation and Western blotting were performed as previously described.32 The following antibodies were used for immunoprecipitation: FKBP51 (sc‐271547; Santa Cruz Biotechnology), StARD13 (HPA035535; Sigma), and Halo (G9281; Promega). The following primary antibodies were used for immunoblotting: FKBP51 (ab126715; Abcam, Cambridge, UK and A301‐430A; Bethyl Laboratories, Inc., Montgomery, TX, USA), StARD13 (HPA035535; Sigma), Halo (G9281; Promega), and β‐actin (Sigma). Horseradish peroxidase‐conjugated secondary antibodies (Amersham Biosciences, Amersham, UK) were used to detect immunoreactive proteins by enhanced chemiluminescence (Thermo Scientific, Waltham, MA, USA).
Stard13
Manufactured by Merck Group
Sourced in United States
StARD13 is a laboratory product manufactured by Merck Group. It functions as a protein involved in the regulation of cholesterol and lipid metabolism. The core function of StARD13 is to facilitate the transport and transfer of lipids within cells.
Automatically generated - may contain errors
1 protocol using stard13
1
Immunoprecipitation and Western Blotting
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!