Mesencult osteogenic stimulatory kit mouse

Manufactured by STEMCELL

Sourced in Canada

MesenCult™ Osteogenic Stimulatory Kit (Mouse) is a laboratory product designed to promote the osteogenic differentiation of mouse mesenchymal stem/stromal cells (MSCs) in vitro. The kit includes the necessary components to support the osteogenic differentiation process.

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Lab products found in correlation

Mesencult adipogenic differentiation kit mouse, by STEMCELL (2 mentions) Alizarin red, by Thermo Fisher Scientific (1 mentions) Oil red o, by Merck Group (1 mentions) Alizarin red, by Muto Pure Chemicals (1 mentions)

Close spelling variants of this product

MesenCult basal medium + mouse MesenCult osteogenic stimulatory kit MesenCult Osteogenic Stimulatory Kit

3 protocols using mesencult osteogenic stimulatory kit mouse

Characterization of Murine Mesenchymal Stromal Cells

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Mesenchymal stromal cells were procured from mouse bone marrow as previously described [21 ,22 ]. Isolated BMC were cultured in a Dulbecco's modified Eagle's medium (DMEM) and plastic-adherent stromal cells were cultivated and used within up 5 passages.
We evaluated differentiation of BMC into adipocytes (MesenCult™ Adipogenic Differentiation Kit (Mouse): STEMCELL technologies, BC, Canada), osteocytes (MesenCult™ Osteogenic Stimulatory Kit (Mouse); STEMCELL technologies), and chondrocytes (Human/Mouse StemXVivo^TM Chondrogenic Media; R&D systems, MN, USA) according to manufacturer's instructions.
Flow cytometry was used to confirm the BMC population via surface marker expression as per established protocols [21 ,22 ]. All antibody details are listed in Table 1.

Taniguchi D., Ahmadipour M., Eiliazadeh A.L., Duchesneau P., Nagayasu T., Haykal S., Karoubi G, & Waddell T.K. (2023). Mesenchymal cells support the early retention of primary alveolar type 2 cells on acellular mouse lung scaffolds. Regenerative Therapy, 25, 92-100.

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Adipogenic and Osteogenic Differentiation of ADSCs and iADSCs

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ADSCs and iADSCs were seeded at 3.2 × 10⁴ cells per well in a Nunc cell-culture- treated 4-well dish (ThermoFisher Scientific, Waltham, MA, USA). After 2 days, the adipogenic differentiation was induced by incubation in a MesenCult Adipogenic Differentiation Kit (Mouse) (STEMCELL Technologies). After 4 and 7 days, cells were stained with Oil Red O (Sigma-Aldrich, St Louis, MO, USA) to determine the presence of intracellular lipid droplets, and the expression of differentiation markers after 7 days of differentiation was examined using real-time quantitative PCR (RT-qPCR). Osteogenic differentiation of ADSCs and iADSCs was induced by incubation in a MesenCult Osteogenic Stimulatory Kit (Mouse) (STEMCELL Technologies) for 11–21 days. Cells after 21 days of differentiation were then stained with Alizarin red (MUTO PURE CHEMICALS, Tokyo, Japan) to detect Ca²⁺ deposits. Cells after 11–21 days of differentiation were also examined for expression of differentiation markers via RT-qPCR. For Alizarin red staining, Nunc cell-culture-treated 4-well dishes (ThermoFisher Scientific) were coated with 0.01% type I collagen (C4243, Sigma-Aldrich).

Ichise T., Ichise H, & Shimizu Y. (2024). Development of a Mouse Experimental System for the In Vivo Characterization of Bioengineered Adipose-Derived Stromal Cells. Cells, 13(7), 582.

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Isolation and Culturing of Primary MSCs and ECs

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Primary MSCs were isolated according to previously published methods [17 ]. For MSC preparation, CD45^-Ter119^- cells were obtained from compact mouse bone with an MSC enrichment kit (Stem Cell Technologies, Vancouver, BC, Canada). Then, the cells were grown in a MesenCult™ Osteogenic Stimulatory Kit (Mouse) (Stem Cell Technologies). Primary ECs were purchased from Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd. (ZQ0446; Shanghai, China). All experiments were performed on fresh, low-passage (p2-3) cells.

Tang Y., Luo K., Chen Y., Chen Y., Zhou R., Chen C., Tan J., Deng M., Dai Q., Yu X., Liu J., Zhang C., Wu W., Xu J., Dong S, & Luo F. (2021). Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering. Bioactive Materials, 6(7), 2039-2057.

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