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Tom20 mcherry

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TOM20-mCherry is a plasmid that encodes a fusion protein consisting of the translocase of outer membrane 20 (TOM20) protein and the mCherry fluorescent protein. TOM20 is a protein involved in the import of proteins into the mitochondria. The mCherry fluorescent protein allows for the visualization of the localization of the TOM20 protein within the cell.

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Lab products found in correlation

Mcherry sec61β, by Addgene (1 mentions) Mito bfp, by Addgene (1 mentions)

2 protocols using tom20 mcherry

1

Fluorescent Protein-Labeled Organelle Markers

Cited 1 time
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EGFP-ORP5A, EGFP-ORP8, EGFP-ORP5ΔPH, EGFP-ORP5ΔTM, EGFP-ORD5, and EGFP-ORD8 were described in Galmes et al. (2016) (link). mCh-Plin1 was described in Ajjaji et al. (2019) (link) and YFP-Seipin was described in Santinho et al. (2020) (link). GFP-Sec22b and RFP-Sec22b were described in Gallo et al. (2020) (link). GFP-Sec61β and ssHRP-KDEL were kindly gifted by T. Rapoport (Harvard University, Cambridge, MA) and D. Cutler (Schikorski et al., 2007 (link)), respectively. Mito-BFP (plasmid #49151; Addgene; http://n2t.net/addgene:49151), Seipin-EGFP (plasmid #129719; Addgene; http://n2t.net/addgene:129719), mCherry-Sec61β (plasmid # 90994; Addgene; https://www.addgene.org/90994) and TOM20-mCherry (plasmid #55146; Addgene; http://n2t.net/addgene:55146) were purchased from Addgene.
Guyard V., Monteiro-Cardoso V.F., Omrane M., Sauvanet C., Houcine A., Boulogne C., Ben Mbarek K., Vitale N., Faklaris O., El Khallouki N., Thiam A.R, & Giordano F. (2022). ORP5 and ORP8 orchestrate lipid droplet biogenesis and maintenance at ER–mitochondria contact sites. The Journal of Cell Biology, 221(9), e202112107.
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2

Mitochondrial Protein Expression Constructs

Cited 6 times
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POCT-PA-GFP and MAPL-FLAG were obtained from H. McBride (McGill University, Canada). P2A-mCherry (84329), TOM20-mCherry (55146), and GFP-OMP25 (141150) were obtained from Addgene. Lentiviral cloning vectors, pWXLd-Ires-Hygro, psPAX2, and pMD2.G were obtained from M. Zeviani (MRC Mitochondrial Biology Unit, Cambridge). Myc-DDK–tagged MTFR1L (RC200212) was obtained from OriGene. The following constructs were already described: pCAG-TdTomato (69 (link)), pSCV2 (pCAG-mVenus) (70 (link)), pCAG-mtDsRed (71 (link)), pCAG-mTAGBFP2 (54 (link)), pCAG-mtYFP (69 (link)), pCAG-mtmTAGBFP2 (69 (link)), pCAG-ActA-mCherry (72 (link)), and pcDNA3.1/Puro-CAG-VSFP-CR (73 (link)).
Tilokani L., Russell F.M., Hamilton S., Virga D.M., Segawa M., Paupe V., Gruszczyk A.V., Protasoni M., Tabara L.C., Johnson M., Anand H., Murphy M.P., Hardie D.G., Polleux F, & Prudent J. (2022). AMPK-dependent phosphorylation of MTFR1L regulates mitochondrial morphology. Science Advances, 8(45), eabo7956.
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