Rat anti mouse cd45 apc

Manufactured by BioLegend

Rat anti–mouse CD45–APC is a monoclonal antibody that binds to the mouse CD45 cell surface antigen. CD45 is a transmembrane protein tyrosine phosphatase expressed on all nucleated hematopoietic cells.

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Lab products found in correlation

Efluor 506, by Thermo Fisher Scientific (1 mentions) 123count ebeads, by Thermo Fisher Scientific (1 mentions) Pharm lyse buffer, by BD (1 mentions) Rat anti mouse cd11b apc cy7, by BioLegend (1 mentions) Facsymphony a5 laser analyzer, by BD (1 mentions) Rat anti mouse cd11c pe cy7, by BD (1 mentions) Fitc rat anti mouse cd45, by BioLegend (1 mentions) Fcr blocking reagent, by Miltenyi Biotec (1 mentions) Countbright absolute counting beads, by Thermo Fisher Scientific (1 mentions)

2 protocols using rat anti mouse cd45 apc

Murine Lung Cell Characterization

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Mouse lung was digested and single-cell suspensions were prepared as previously described (46 (link)). Cell suspensions underwent red blood cell lysis using Pharm Lyse buffer (BD Biosciences). Live/dead staining was performed in protein-free solution (HBSS) using fixable viability dye eFluor 506 (eBioscience), followed by incubation with FcR-blocking reagent (Miltenyi Biotec). Antibodies utilized for murine cell staining included rat anti–mouse CD45–FITC (BioLegend, 30-F11), rat anti–mouse Ly6C–eFluor450 (eBiosciences, HK1.4), rat anti–mouse I-A/I-E–PerCPCy5.5 (BioLegend, M5/114.15.2), rat anti–mouse CD45–APC (BioLegend, 30-F11), rat anti–mouse Ly6G–Alexa Fluor 700 (BioLegend, 1A8), rat anti–mouse NK1.1–Alexa Fluor 700 (BD, PK136), rat anti–mouse CD11b–APCCy7 (BioLegend, M1/70), rat anti–mouse CD64–PE (BioLegend, X54-5/7.1), rat anti–mouse SiglecF–PECF594 (BD, E50-2440), and rat anti–mouse CD11c–PECy7 (BD, HL3). For neutrophil quantification, 123Count eBeads (Invitrogen) were added. Flow analysis of fixed samples was done on a BD FACSymphony A5-Laser Analyzer at the Northwestern University Robert H. Lurie Comprehensive Cancer Center Flow Cytometry Core facility. Acquired data were analyzed with FlowJo v10.8 (FlowJo).

Yang W., Cerier E.J., Núñez-Santana F.L., Wu Q., Yan Y., Kurihara C., Liu X., Yeldandi A., Khurram N., Avella-Patino D., Sun H., Budinger G.R., Kreisel D., Mohanakumar T., Lecuona E, & Bharat A. (2022). IL-1β–dependent extravasation of preexisting lung-restricted autoantibodies during lung transplantation activates complement and mediates primary graft dysfunction. The Journal of Clinical Investigation, 132(20), e157975.

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Quantification of Splenic and Bone Marrow B Cells

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Single cell suspensions of immune cells were prepared from spleens and bone marrow (femur) as previously described (Li et al., 2019 (link); Rohatgi et al., 2018 (link)). Cells were treated with Fc Block and then stained with rat anti-mouse CD45-APC (1:200, clone 30-F11; BioLegend), rat anti-mouse CD19-APC/Fire750 (1:300, clone 6D5; BioLegend), and rat anti-mouse B220-PE (1:300, clone RA3–6B2; BioLegend) as previously described (Li et al., 2019 (link); Rohatgi et al., 2018 (link)). Events were acquired on a BD X20 flow cytometer, using CountBright Absolute Counting Beads (ThermoFisher) according to the manufacturer’s protocol, except that 25 μL was used per sample. B cells were defined as CD45+ CD19+ B220+ cells.

Zou W., Rohatgi N., Brestoff J.R., Li Y., Barve R.A., Tycksen E., Kim Y., Silva M.J, & Teitelbaum S.L. (2020). Ablation of Fat Cells in Adult Mice Induces Massive Bone Gain. Cell metabolism, 32(5), 801-813.e6.

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