Log In Sign up
The largest database of trusted experimental protocols

Hladr g46 6 apc h7

Manufactured by BD
Visit Supplier

HLADR (G46-6) APC-H7 is a monoclonal antibody product designed for flow cytometry applications. It is used to identify and characterize human leukocyte antigen DR (HLA-DR) expression on cells. The APC-H7 fluorochrome conjugated to the antibody allows for multicolor flow cytometry analysis.

Automatically generated - may contain errors

Lab products found in correlation

Cd3 clone sp34 2 al700, by BD (2 mentions) Il 2 mq1 17h12 fitc, by BD (2 mentions) Ki67 b56 fitc, by BD (2 mentions) Cd4 okt4 efluor450, by BD (2 mentions) Tnfa mab11 pe cy7, by Thermo Fisher Scientific (2 mentions) Cd28 cd28.2 ecd, by Beckman Coulter (2 mentions) Cd95 dx2 pe cy5, by BioLegend (2 mentions) Live dead fixable aqua dead cell stain, by Thermo Fisher Scientific (2 mentions) Lsrfortessa, by BD (2 mentions) Cytofix cytoperm, by BD (2 mentions) Facsaria, by BD (2 mentions)

2 protocols using hladr g46 6 apc h7

1

Multiparametric Cytometry of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Polychromatic flow cytometry and cell sorting were performed on stained mononuclear cells utilizing the BD LSRFortessa and FACS Aria, respectively, equipped with FACS DiVA software. The following Abs were used for staining at predetermined concentrations: CD3 (clone SP34-2) AL700, HLADR (G46-6) APC-H7, IL-2 (MQ1-17H12) FITC, and Ki67 (B56) FITC from BD; CD4 (OKT4) eFluor450, CD8 (SK1) PerCP-e710, IFNg (4S.B3) eFluor450, IL17A (eBio64DEC17) PE, IL22 (IL22JOP) APC, and TNFa (MAb11) PE-Cy7 from eBioscience; CD28 (CD28.2) ECD from Beckman Coulter; and CD95 (DX2) PE-Cy5 from Biolegend. Cell viability was assessed using the Live/Dead Aqua Fixable Dead Cell Stain (Invitrogen). Cells were permeabilized with Cytofix/Cytoperm (BD) prior to intracellular staining. Acquired data were analyzed using FlowJo software. To quantify cell subset frequencies, we used a threshold cutoff of 100 cells of the parent population. For TH17 functional analyses, cytokine gates were established on a threshold cutoff of 100 CD4+ or CD8+ TM and Boolean gates utilized to ascertain frequencies – populations not meeting this threshold were not considered for analysis.
Ortiz A.M., Klase Z.A., DiNapoli S.R., Vujkovic-Cvijin I., Carmack K., Perkins M.R., Calantone N., Vinton C.L., Riddick N.E., Gallagher J., Klatt N.R., McCune J.M., Estes J.D., Paiardini M, & Brenchley J.M. (2015). IL-21 and Probiotic Therapy Improve TH17 Frequencies, Microbial Translocation, and Microbiome in ARV-Treated, SIV-Infected Macaques. Mucosal immunology, 9(2), 458-467.
+ Open protocol
+ Expand
2

Multiparametric Cytometry of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Polychromatic flow cytometry and cell sorting were performed on stained mononuclear cells utilizing the BD LSRFortessa and FACS Aria, respectively, equipped with FACS DiVA software. The following Abs were used for staining at predetermined concentrations: CD3 (clone SP34-2) AL700, HLADR (G46-6) APC-H7, IL-2 (MQ1-17H12) FITC, and Ki67 (B56) FITC from BD; CD4 (OKT4) eFluor450, CD8 (SK1) PerCP-e710, IFNg (4S.B3) eFluor450, IL17A (eBio64DEC17) PE, IL22 (IL22JOP) APC, and TNFa (MAb11) PE-Cy7 from eBioscience; CD28 (CD28.2) ECD from Beckman Coulter; and CD95 (DX2) PE-Cy5 from Biolegend. Cell viability was assessed using the Live/Dead Aqua Fixable Dead Cell Stain (Invitrogen). Cells were permeabilized with Cytofix/Cytoperm (BD) prior to intracellular staining. Acquired data were analyzed using FlowJo software. To quantify cell subset frequencies, we used a threshold cutoff of 100 cells of the parent population. For TH17 functional analyses, cytokine gates were established on a threshold cutoff of 100 CD4+ or CD8+ TM and Boolean gates utilized to ascertain frequencies – populations not meeting this threshold were not considered for analysis.
Ortiz A.M., Klase Z.A., DiNapoli S.R., Vujkovic-Cvijin I., Carmack K., Perkins M.R., Calantone N., Vinton C.L., Riddick N.E., Gallagher J., Klatt N.R., McCune J.M., Estes J.D., Paiardini M, & Brenchley J.M. (2015). IL-21 and Probiotic Therapy Improve TH17 Frequencies, Microbial Translocation, and Microbiome in ARV-Treated, SIV-Infected Macaques. Mucosal immunology, 9(2), 458-467.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!