Cytometric bead array mouse cytokine kit

Manufactured by BD

Sourced in United States

The Cytometric Bead Array (CBA) mouse cytokine kit is a laboratory equipment used to quantitatively measure multiple mouse cytokines in a single sample. The kit utilizes flow cytometry technology to detect and analyze the concentration of various cytokines simultaneously.

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Lab products found in correlation

Dy460, by R&D Systems (2 mentions) Cell lysis buffer, by Cell Signaling Technology (2 mentions) Precellys tissue homogenizer tubes, by Bertin Technologies (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) N n dimethylformamide (dmf), by Merck Group (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Fcap array, by BD (1 mentions) Lsr 2, by BD (1 mentions) Facscalibur flow cytometer, by BD (1 mentions)

8 protocols using cytometric bead array mouse cytokine kit

Cytokine Concentration Analysis

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Cytokine concentrations were measured using a BD Cytometric Bead Array (CBA) mouse cytokine kit according to manufacturer’s protocols.

Opejin A., Surnov A., Misulovin Z., Pherson M., Gross C., Iberg C.A., Fallahee I., Bourque J., Dorsett D, & Hawiger D. (2020). A Two-Step Process of Effector Programming Governs CD4+ T Cell Fate Determination Induced by Antigenic Activation in the Steady State. Cell reports, 33(8), 108424.

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Signaling Pathway Protein Detection

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Sigma Chemical Co. (St. Louis, MO) provided DMF, LPS (Escherichia coli 055:B5), and dimethylsulfoxide (DMSO). Invitrogen Gibco (Grand Island, NY) provided penicillin, streptomycin, fetal bovine serum (FBS), and Dulbecco's modified Eagle's medium (DMEM). Polyclonal antibodies against phospho-p44/42 MAPK (Erk1/2), IKKα, and IκBα, monoclonal antibodies against phosphor-IKKα/β, phosphor-IκBα, NF-κb p65, and phosphor-NF-κB p65 were supplied by the Cell Signaling Technology Inc. (Beverly, MA). Polyclonal antibodies against GAPDH, iNOS, and ERK1 were bought from Santa Cruz Biotechnology (CA). GE Healthcare (Buckinghamshire, UK) provided goat–mouse secondary antibodies as well as HRP-conjugated anti-rabbit. We bought polyvinylidenefluoride (PVDF) membrane from Whatman GmbH (Germany). Thermo Fisher Scientific Inc. (Pierce, Rockford) provided Pierce ECL western blotting substrate. The Cytometric Bead Array (CBA) Mouse Cytokine Kit was purchased from BD (Becton, Dickinson and Company, USA). The rest of the chemicals were of reagent grade.

Fang H., Wang X., Damarla M., Sun R., He Q., Li R., Luo P., Liu J.O, & Xia Z. (2023). Dimethyl Fumarate Protects against Lipopolysaccharide- (LPS-) Induced Sepsis through Inhibition of NF-κB Pathway in Mice. Mediators of Inflammation, 2023, 5133505.

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Cytokine Concentration Analysis

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Cytokine concentrations were measured using a BD Cytometric Bead Array (CBA) mouse cytokine kit according to manufacturer’s protocols.

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Cytokine Profiling in Kidney Lysates

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Tumor necrosis factor-α, IL-6, IL-10, and IL-4 levels from kidney lysates were analyzed using BD cytometric bead array mouse cytokine kit following the manufacturer's instructions (BD Bioscience, Franklin Lakes, New Jersey, USA). Data were analyzed by BD FCAP Array (BD Bioscience).

Rao P., Qiao X., Hua W., Hu M., Tahan M., Chen T., Yu H., Ren X., Cao Q., Wang Y., Yang Y., Wang Y.M., Lee V.W., Alexander S.I., Harris D.C, & Zheng G. (2021). Promotion of β-Catenin/Forkhead Box Protein O Signaling Mediates Epithelial Repair in Kidney Injury. The American Journal of Pathology, 191(6), 993-1009.

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Cytokine Profiling in Intestinal Tissue

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Intestinal tissue was weighed and homogenized (100 mg tissue/mL) in cell lysis buffer (Cell Signaling Technology, Beverly, MA) with protease inhibitors (Roche) using Precellys tissue homogenizer tubes (Bertin Technologies, Montigny, France). For cytokine bead array, the BD Cytometric Bead Array Mouse Cytokine Kit (560485; BD Biosciences) was used according to the manufacturer’s protocol. Concentrations in tissue lysates were corrected for total protein content as measured by BCA (Pierce, Thermo Fisher Scientific). The concentration of cytokines were determined by sandwich enzyme-linked immunoassay kit (DY460; R&D Systems, Minneapolis, MN).

Meijer B.J., Giugliano F.P., Baan B., van der Meer J.H., Meisner S., van Roest M., Koelink P.J., de Boer R.J., Jones N., Breitwieser W., van der Wel N.N., Wildenberg M.E., van den Brink G.R., Heijmans J, & Muncan V. (2020). ATF2 and ATF7 Are Critical Mediators of Intestinal Epithelial Repair. Cellular and Molecular Gastroenterology and Hepatology, 10(1), 23-42.

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Cytokine Quantification Protocol

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The levels of serum cytokines were determined using the Cytometric Bead Array Mouse Cytokine kit (BD Biosciences). Mouse cytokine-specific bead sets and standards were implemented as per the manufacturer’s instructions. The fluorescence produced by the beads was measured using BD LSR II and analyzed with FCAP Array software as per manufacturer instructions.

Kumari R., Palaniyandi S., Strattan E., Huang T., Kohler K., Jabbour N., Dalland J., Du J., Kesler M.V., Chen Y.H, & Hildebrandt G.C. (2020). TNFAIP8 Deficiency Exacerbates Acute Graft Versus Host Disease in a Murine Model of Allogeneic Hematopoietic Cell Transplantation. Transplantation, 104(3), 500-510.

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Cytokine Profiling Using Bead Array

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The levels of serum cytokines were determined using the Cytometric Bead Array Mouse Cytokine kit (BD Biosciences, MD). Mouse cytokine-specific bead set and standards were implemented as per the manufacturer’s instructions. The fluorescence produced by the beads was measured using FACSCalibur flow cytometer (BD Biosciences, San Jose, CA) and analyzed with the accompanying software.

Palaniyandi S., Kumari R., Radhakrishnan S.V., Strattan E., Hakim N., Munker R., Kesler M.V, & Hildebrandt G.C. (2020). The Prolyl Hydroxylase Inhibitor Dimethyl Oxalyl Glycine Decreases Early Gastrointestinal GVHD in Experimental Allogeneic Hematopoietic Cell Transplantation. Transplantation, 104(12), 2507-2515.

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Intestinal Tissue Homogenization and Cytokine Assays

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Intestinal tissue was weighed and homogenized (100 mg tissue/mL) in cell lysis buffer (Cell Signaling Technology, Beverly, MA) with protease inhibitors (Roche) using Precellys tissue homogenizer tubes (Bertin Technologies, Montigny, France). For cytokine bead array, the Cytometric Bead Array Mouse Cytokine Kit (560485; BD Biosciences) was used. Serum amyloid A and myeloperoxidase (MPO) concentration were measured by enzyme-linked immunosorbent assay kits for MPO (HK210-02; Hycult Biotech, Uden, Netherlands) and serum amyloid A (TP 802M; Tridelta Development, Ltd, Maynooth, Ireland). MPO concentrations in tissue lysates were corrected for total protein content as measured by bicinchoninic acid (BCA) assay (Pierce, Thermo Fisher Scientific). The concentration of CXCL12 in the supernatant of fibroblasts was determined using a sandwich enzyme-linked immunosorbent assay kit (DY460; R&D Systems).

Westendorp B.F., Büller N.V., Karpus O.N., van Dop W.A., Koster J., Versteeg R., Koelink P.J., Snel C.Y., Meisner S., Roelofs J.J., Uhmann A., Ver Loren van Themaat E., Heijmans J., Hahn H., Muncan V., Wildenberg M.E, & van den Brink G.R. (2017). Indian Hedgehog Suppresses a Stromal Cell–Driven Intestinal Immune Response. Cellular and Molecular Gastroenterology and Hepatology, 5(1), 67-82.e1.

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