Specimens were plunge-frozen onto a Quantifoil R2/2, Cu/Rh holey carbon grid using an FEI Vitrobot (FEI Company, USA) and imaged with an FEI Tecnai G2 Polara electron microscope operating at 300 kV and equipped with a Gatan energy filter and 4 × 4 k Gatan Ultrascan CCD camera (binned to 2 × 2 k). For tomography, samples were imaged using an FEI Titan Krios TEM operating at 300 kV, equipped with a 4k × 4k K2 Summit direct electron detector at a magnification of 26 k, corresponding to a pixel size of 0.45 nm (for K2) at the specimen level. Tilt series were acquired using SerialEM software 58 (link) between ± 55° with 1° increment. The defocus was set to between 5 and 8 μm, and the total dose for each tilt series was around 120 e/Å2. Tilt series was aligned using the fiducial markers and IMOD software package and reconstruction was computed using simultaneous iterative reconstruction technique (SIRT) 59 (link),60 (link).
Tecnai g2 polara electron microscope
Manufactured by Ametek
Sourced in United States
The Tecnai G2 Polara electron microscope is a high-resolution imaging system designed for advanced structural and analytical studies. It features a field emission gun, providing a stable and bright electron beam for high-resolution imaging. The microscope is capable of operating at accelerating voltages up to 300 kV, enabling the visualization of a wide range of samples at the nanoscale level.
Automatically generated - may contain errors
2 protocols using tecnai g2 polara electron microscope
1
Cryo-EM Imaging and Tomography
2
Cryo-EM Imaging and Tomography
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Specimens were plunge-frozen onto a Quantifoil R2/2, Cu/Rh holey carbon grid using an FEI Vitrobot (FEI Company, USA) and imaged with an FEI Tecnai G2 Polara electron microscope operating at 300 kV and equipped with a Gatan energy filter and 4 × 4 k Gatan Ultrascan CCD camera (binned to 2 × 2 k). For tomography, samples were imaged using an FEI Titan Krios TEM operating at 300 kV, equipped with a 4k × 4k K2 Summit direct electron detector at a magnification of 26 k, corresponding to a pixel size of 0.45 nm (for K2) at the specimen level. Tilt series were acquired using SerialEM software 58 (link) between ± 55° with 1° increment. The defocus was set to between 5 and 8 μm, and the total dose for each tilt series was around 120 e/Å2. Tilt series was aligned using the fiducial markers and IMOD software package and reconstruction was computed using simultaneous iterative reconstruction technique (SIRT) 59 (link),60 (link).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!