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Jem 1400 plus 120 kv

Manufactured by JEOL
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The JEM-1400 plus 120 kV is a transmission electron microscope (TEM) produced by JEOL. It operates at an accelerating voltage of 120 kV and is designed for high-resolution imaging and analysis of a wide range of materials and specimens.

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Lab products found in correlation

Nano s90, by Malvern Panalytical (1 mentions)

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JEM-1400 (1333 citations) JEOL 1400 Plus (76 citations) JEM-1400 120 kV (6 citations) JEM-1400(PLUS) 120 kV transmission electron microscope (4 citations)

3 protocols using jem 1400 plus 120 kv

1

Visualizing Tacrolimus Nanocarriers by TEM

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Tacrolimus NCs were visualized by transmission electron microscopy (TEM) using a JEM-1400 plus 120 kV (JEOL Ltd., Tokyo, Japan) apparatus. Specimens were mixed with uranyl acetate for negative staining and dried at room temperature.
Rebibo L., Tam C., Sun Y., Shoshani E., Badihi A., Nassar T, & Benita S. (2021). Topical Tacrolimus Nanocapsules Eye Drops for Therapeutic Effect Enhancement in both Anterior and Posterior Ocular Inflammation Models. Journal of controlled release : official journal of the Controlled Release Society, 333, 283-297.
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2

Ultrastructural Analysis of Skeletal Muscle

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Skeletal muscle was immediately fixed in 2% glutaraldehyde and 2% paraformaldehyde in 0.1 M PBS (pH 7.4). After washing with PBS, the tissues were post-fixed with 1% osmium tetroxide and washed again with PBS. The tissues were then embedded in pure Epon 812 mixture after dehydration in ethanol series and infiltration in a propylene oxide:epon mixture series. Ultrathin sections (~70 nm) were obtained with a model MT-X ultramicrotome (RMC, Tucson, AZ, USA) and then stained with 2% uranyl acetate and lead citrate. The sections were visualized by Cryo-TEM (JEM-1400 Plus, 120 kV) (Jeol Ltd., Tokyo, Japan).
Lee D.H., Ahn J., Jang Y.J., Seo H.D., Ha T.Y., Kim M.J., Huh Y.H, & Jung C.H. (2020). Withania somnifera Extract Enhances Energy Expenditure via Improving Mitochondrial Function in Adipose Tissue and Skeletal Muscle. Nutrients, 12(2), 431.
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3

Polymer-coated Hydrophilic Carboxylic Iron Oxide Nanoparticles

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Polymer-coated HCIONPs were prepared as previously described by our lab. Briefly, HCIONPs (~15 nm diameter) were synthesized in organic solvent by thermal decomposition [42 ]. Single core nanocrystals were rendered soluble in aqueous solution after coating with diblock copolymer poly(ethylene oxide)-b-poly(γ-methacryloxypropyl trimethoxysilane) (PEO-b-PγMPS), which is synthesized by reversible addition fragmentation chain transfer (RAFT) polymerization, and purifying by a magnetic separator (Frantz laboratory) [42 ,43 (link)]. The purified, polymer-coated HCIONPs were viewed by transmission electron microscopy (TEM) (JEOL JEM-1400Plus 120 kV). Hydrodynamic size and zeta potential were determined by dynamic light scattering (DLS) on a Zetasizer Nano S90 (Malvern). The HCIONP iron concentration was determined using o-phenanthroline as previously described [42 ]. HCIONPs were diluted in phosphate buffered saline (PBS) for in vitro and in vivo studies.
Paholak H.J., Stevers N.O., Chen H., Burnett J.P., He M., Korkaya H., McDermott S.P., Deol Y., Clouthier S.G., Luther T., Li Q., Wicha M.S, & Sun D. (2016). Elimination of epithelial-like and mesenchymal-like breast cancer stem cells to inhibit metastasis following nanoparticle-mediated photothermal therapy. Biomaterials, 104, 145-157.
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