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Epiquik m6a methylation quantification kit

Manufactured by Epigentek
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The EpiQuik m6A methylation quantification kit is a lab equipment product from Epigentek that provides a simple and fast method for quantifying global N6-methyladenosine (m6A) levels in RNA samples. The kit uses a unique capture and detection antibody-based colorimetric assay format to measure m6A abundance.

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Lab products found in correlation

Ne per nuclear and cytoplasmic extraction reagent, by Thermo Fisher Scientific (1 mentions) Tri reagent, by Molecular Research Center (1 mentions) Epigenase m6a methylase activity inhibition assay kit, by Epigentek (1 mentions) Actinomycin d, by GLPBIO (1 mentions) Nanodrop 1000, by Thermo Fisher Scientific (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

EpiQuik m6A RNA Methylation Quantification Kit (150 citations) EpiQuik m6A RNA Methylation Quantification kit (colorimetric) (12 citations) EpiQuik m6A/m RNA Methylation Quantification Kit (4 citations) EpiQuik fluorometric m6A RNA methylation quantification kit (3 citations) EpiQuikTM (2 citations) EpiQuik m6A RNA Methylation Quantification ELISA kit (2 citations)

6 protocols using epiquik m6a methylation quantification kit

1

Quantifying m6A Methylation in METTL3 and CPSF30 KD Parasites

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Total RNA was isolated from purified METTL3 KD and CPSF30 KD parasites that were treated with vehicle or IAA and the amount of m6A modification was assessed from total RNA using the colorimetric EpiQuik m6A methylation quantification kit as per manufacturer’s instructions (EpiGentek, P-9005–96).
Farhat D.C., Bowler M.W., Communie G., Pontier D., Belmudes L., Mas C., Corrao C., Couté Y., Bougdour A., Lagrange T., Hakimi M.A, & Swale C. (2021). A plant-like mechanism coupling m6A reading to polyadenylation safeguards transcriptome integrity and developmental gene partitioning in Toxoplasma. eLife, 10, e68312.
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2

n6-Methyladenosine Methylation Assay

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Sodium arsenite ( NaAsO2 ; #SLBW7984) and FA (#F7876) were obtained from Sigma. SAM (#B9003S) was obtained from New England Biolabs. Actinomycin D (#GC16866) was obtained from GlpBio. Epigenase m6A methylase activity/inhibition assay kit (#P9019-96) and EpiQuik m6A methylation quantification kit (#P-9005-48) were purchased from Epigentek. NE-PER nuclear and cytoplasmic extraction reagents (#78835) were purchased from Thermo Scientific. TRI reagent (#TR-118) was purchased from Molecular Research Center, Inc. The cysteine-rich angiogenic inducer 61 (CYR61) ELISA kit (#CSB-E13884h) was purchased from Cusabio.
Song Y.P., Lv J.W., Zhang Z.C., Qian Q.H., Fan Y.J., Chen D.Z., Zhang H., Xu F.X., Zhang C., Huang Y., Wang H., Wei W, & Xu D.X. (2023). Effects of Gestational Arsenic Exposures on Placental and Fetal Development in Mice: The Role of Cyr61. Environmental Health Perspectives, 131(9), 097004.
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3

Quantification of m6A RNA Modification

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Total RNA was isolated from purified parasites that were treated with vehicle or 500 μM IAA as outlined above. The amount of m6A modification was assessed from 300 ng total RNA using the colorimetric EpiQuik m6A methylation quantification kit as per manufacturer’s instructions (EpiGentek, P-9005-96). The m6A levels were determined from three or four biological replicates and significance was assessed using Student’s t-test assuming unequal variances.
Holmes M.J., Padgett L.R., Bastos M.S, & Sullivan WJ J.r. (2021). m6A RNA methylation facilitates pre-mRNA 3’-end formation and is essential for viability of Toxoplasma gondii. PLoS Pathogens, 17(7), e1009335.
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4

Quantifying mRNA m6A Methylation

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Total RNA was isolated as above. The quality of RNA was analyzed using a NanoDrop1000 (Thermo Fisher, United States). The EpiQuik m6A Methylation Quantification Kit (Epigentek, P-9005-96, United States) was used to measure the global m6A enrichment of mRNA. 200 ng RNA was coated in assay wells from each sample. The m6A levels are colorimetrical quantified at a wavelength of 450 nm absorbance.
Li H., Lin J., Cheng S., Chi J., Luo J., Tang Y., Zhao W., Shu Y., Liu X, & Xu C. (2023). Comprehensive analysis of differences in N6-methyladenosine RNA methylomes in Helicobacter pylori infection. Frontiers in Cell and Developmental Biology, 11, 1136096.
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5

Quantification of m6A Methylation in mRNA

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Total RNA was isolated from cardiomyocytes using TRIzol (Invitrogen) according to manufacturer’s instructions. The quality of RNA was analyzed using a NanoDrop (). The global m6A enrichment in mRNA was measured by the EpiQuik m6A Methylation Quantification Kit (Epigentek, P-9005-48, colorimetric) based on manufacturer’s protocol. Each sample RNA (200 ng) was coated in assay wells. M6A antibody (ab195352, 1:1000) in solution was added to the assay wells separately at a suitable dilution concentration. The m6A levels are colorimetrically quantified at a wavelength of 450 nm absorbance.
Ke W.L., Huang Z.W., Peng C.L, & Ke Y.P. (2022). m6A demethylase FTO regulates the apoptosis and inflammation of cardiomyocytes via YAP1 in ischemia-reperfusion injury. Bioengineered, 13(3), 5443-5452.
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6

Quantifying m6A RNA Methylation

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Total RNA was extracted from prostate cancer cells using Trizol. Extracted 200 ng RNA was used by EpiQuik m6A Methylation Quantification Kit (P9005-48) (Epigentek, Farmingdale, NY), and the m6A level of each sample was measured according to the manufacturer’s instructions.
Wang Y., Chen J., Gao W.Q, & Yang R. (2022). METTL14 promotes prostate tumorigenesis by inhibiting THBS1 via an m6A-YTHDF2-dependent mechanism. Cell Death Discovery, 8, 143.
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