Cotinine d3

Manufactured by LGC

Sourced in Canada

Cotinine-d3 is a stable isotope-labeled internal standard used in analytical methods for the detection and quantification of cotinine, a major metabolite of nicotine. It serves as a reference compound to ensure accurate and reliable measurement of cotinine in various biological samples.

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Lab products found in correlation

Nicotine, by Merck Group (1 mentions) Cotinine, by Merck Group (1 mentions) Oasis hlb cartridge, by Waters Corporation (1 mentions) Thc cooh, by Lipomed (1 mentions) Cotinine, by LGC (1 mentions) Mdma d5, by Lipomed (1 mentions) Propylene glycol, by Merck Group (1 mentions) Lc ms grade methanol, by Merck Group (1 mentions) Milli q system, by Merck Group (1 mentions) Etoac, by Merck Group (1 mentions) Ch2cl2, by Merck Group (1 mentions) 13c6 nnal, by LGC (1 mentions)

4 protocols using cotinine d3

Nicotine and Cotinine Standardization

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Nicotine and cotinine were purchased from Sigma-Aldrich Japan (Tokyo, Japan). Nicotine-d₃ (isotopic purity 98.4%), and cotinine-d₃ (isotopic purity 99.9%) purchased from Toronto Research Chemicals Inc. (Toronto, Ontario, Canada) were used as an internal standard (IS). Stock solutions of 1 mg mL⁻¹ of each compound were prepared by dissolving in methanol. The working solutions were prepared by diluting these stock solutions with distilled water to the required concentration. These prepared solutions were stored at 4 °C in refrigerator until use. Methanol and distilled water were of LC–MS grade, while all other chemicals were of analytical reagent grade.

Kataoka H., Kaji S, & Moai M. (2021). Risk Assessment of Passive Smoking Based on Analysis of Hair Nicotine and Cotinine as Exposure Biomarkers by In-Tube Solid-Phase Microextraction Coupled On-Line to LC-MS/MS. Molecules, 26(23), 7356.

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Comprehensive Quantification of Illicit Drugs

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BE, AMPH, METH, MDMA, MOR, THC-COOH, and their deuterated analogues used as internal standards (BE-d₃, MOR-d₃, THC-COOH-d₃, MDMA-d₅) were purchased from Lipomed (Arlesheim, Switzerland). EtS and EtS-d₅ were obtained from Restek (Bellefonte, PA, USA). Cotinine and Cotinine-d₃ were purchased from Toronto Research Chemicals (TRC) (Ontario, Canada). Oasis HLB cartridges (6 mL/500 mg) were obtained from Waters Corporation (Milford, MA, USA). Glass-fiber filters (Whatman® glass microfiber filters, Grade GF/F, 0.7 μm) were purchased from Merck, (Darmstadt, Germany) as well as ammonium acetate, liquid chromatography grade methanol, ultrapure water, acetonitrile, and acetic acid (98 % purity).

, & Guzel E.Y. (2022). Monitoring of changes in illicit drugs, alcohol, and nicotine consumption during Ramadan via wastewater analysis. Environmental Science and Pollution Research International, 29(59), 89245-89254.

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Measurement of E-Cigarette Constituents

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Propylene glycol (PG; >99.5%) and vegetable glycerin (VG; 99.5%), as well as LC-MS grade methanol were purchased from Sigma-Aldrich (Milwaukee, WI). Several brands of e-cigs and e-liquids were purchased locally or online: Set I (blu®: Classic Tobacco, CT; Magnificent Menthol, MM; Vivid Vanilla, VV; Cherry Crush, CC) and Set II (Halo®: Menthol, Mocha Café, Southern Ice). As labeled by vendor, Set I e-cartridges contained 13–16 mg nicotine, whereas Set II e-liquids contained 6 mg/mL nicotine. Ultra-pure water was used in making solutions (Milli-Q system; Millipore Corporation; Bedford, MA). KY reference cigarettes, 3R4F, were purchased from the Center for Tobacco Reference Products (Univ. Kentucky; Lexington, KY). Isotopically-labeled standards (IS) for UPLC-MS/MS analysis: d₃-nicotine, d₃-cotinine, d₃-3-hydroxycotinine (d₃-3HC), d₃-3-hydroxypropyl mercapturic acid (d₃-3HPMA), and d₃-N-Acetyl-S-(3-hydroxypropyl-1-methyl)-L-cysteine (d₃-HPMMA) were purchased from Toronto Research Chemicals (Toronto, Canada).

Conklin D.J., Ogunwale M.A., Chen Y., Theis W.S., Nantz M.H., Fu X.A., Chen L.C., Riggs D.W., Lorkiewicz P., Bhatnagar A, & Srivastava S. (2018). Electronic cigarette-generated aldehydes: The contribution of e-liquid components to their formation and the use of urinary aldehyde metabolites as biomarkers of exposure. Aerosol science and technology : the journal of the American Association for Aerosol Research, 52(11), 1219-1232.

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Analytical Standards for Tobacco Biomarkers

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Analytical standards for NNAL, NNN, cotinine and their respective isotope-labeled analogues [¹³C₆]NNAL, ¹³C₆NNN, and [D₃]cotinine were obtained from Toronto Research Chemicals, Inc. (Canada). β-Glucuronidase (Type IX from E. coli) was purchased from Sigma Aldrich Chemicals (Bangalore, India). Diatomaceous Earth Chem Elut (5 mL unbuffered) cartridges and Bond Elut 1 cc (100 mg, LRC-SI) cartridges were purchased from Agilent Technologies (Bangalore, India). Oasis MCX 1 cc (30 mg) extraction cartridges were obtained from Waters (Bangalore, India). CH₂Cl₂, EtOAc, MeOH, and other sample preparation reagents were purchased either from Sigma Aldrich, Fisher scientific or Sisco Research Laboratories (Mumbai, India).

Nikam S.S., Gurjar M., Singhavi H., Patil A., Singh A., Villalta P., Chaturvedi P., Khariwala S.S., Gota V, & Stepanov I. (2021). Simultaneous analysis of urinary total 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol, N′-nitrosonornicotine, and cotinine by liquid chromatography-tandem mass-spectrometry. Scientific Reports, 11, 20007.

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